ER Recombinant antibody, PBS Only

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  WB analysis using 84564-4-RR (same clone as 84564-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84564-4-RR (ER antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84564-4-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 84564-4-RR (same clone as 84564-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84564-4-RR (ER antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84564-4-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 84564-4-RR (same clone as 84564-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ER antibody (84564-4-RR, Clone: 241825D5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84564-4-PBS in a different storage buffer formulation.

ER {ptg:Brand} Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 84564-4-PBS

Clone No.241825D5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ESR1, ESR, ERα, ER-alpha, Era

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Product Information

84564-4-PBS targets ER in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: estrogen receptor 1
• Calculated Molecular Weight: 595 aa, 66 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC128573
• Gene Symbol: ER
• Gene ID (NCBI): 2099
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P03372
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

The estrogen receptor (ESR, ER) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. ESR1, also known as ESR or NR3A1, belongs to the nuclear hormone receptor family and NR3 subfamily. It is a nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. ESR1 can activate the transcriptional activity of TFF1.[PMID: 11731608,10970861]