EPRS Polyclonal antibody

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  WB analysis using 25307-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25307-1-AP (EPRS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 25307-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25307-1-AP (EPRS Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 25307-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using EPRS antibody (25307-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

EPRS Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 25307-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

EPRS1, GLNS, EARS, Cell proliferation-inducing gene 32 protein, Bifunctional glutamate/proline--tRNA ligase

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Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells, HeLa cells
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

25307-1-AP targets EPRS in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EPRS fusion protein Ag18011
• Full Name: glutamyl-prolyl-tRNA synthetase
• Calculated Molecular Weight: 1512 aa, 171 kDa
• Observed Molecular Weight: 171 kDa
• GenBank Accession Number: BC126275
• Gene Symbol: EPRS
• Gene ID (NCBI): 2058
• RRID: AB_2880023
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P07814
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human EPRS is a 172 kDa, 1512 amino acid polypeptide consisting of three major domains. The N and C termini contain ERS and PRS catalytic domains, respectively, joined by a 300 amino acid linker containing three tandem WHEP-TRS (referred to as WHEP) domains. EPRS is a bifunctional aminoacyl-tRNA synthetase that catalyzes the aminoacylation of glutamic acid and proline tRNA species. EPRS has a special role in GAIT-mediated translational control, as it is solely responsible for recognition and interaction with GAIT elements in target mRNAs. (PMID: 29576217, PMID: 22386318, PMID: 19647514)

Publications

Species	Application	Title
human	WB	Proteomics; Motile hepatocellular carcinoma cells preferentially secret sugar metabolism regulatory proteins via exosomes.; Authors - Jing Zhang
human	WB	Nat Commun; Phenylalanine-tRNA aminoacylation is compromised by ALS/FTD-associated C9orf72 C4G2 repeat RNA; Authors - Mirjana Malnar Črnigoj