EPN3 Polyclonal antibody

EPN3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 20178-1-AP

Synonyms

Epsin-3, EPS-15-interacting protein 3

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Validation Data Gallery

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  WB analysis using 20178-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20178-1-AP (EPN3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 20178-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 20178-1-AP (EPN3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: MCF-10A cells, MCF-7 cells, mouse stomach tissue, rat stomach tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20178-1-AP targets EPN3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EPN3 fusion protein Ag14021
• Full Name: epsin 3
• Calculated Molecular Weight: 632 aa, 68 kDa
• Observed Molecular Weight: 68-70 kDa
• GenBank Accession Number: BC001038
• Gene Symbol: EPN3
• Gene ID (NCBI): 55040
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Epsin 3 (EPN3) belongs to the epsin family, which also includes EPN1 and EPN2. They belong to the endocytic clathrin family and are involved in receptor endocytosis. EPN3 is expressed in breast cancer and is an oncogene associated with breast cancer prognosis and treatment (PMID: 34779498;PMID: 32541686).