EPN2 Polyclonal antibody

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  WB analysis using 31334-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31334-1-AP (EPN2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse lung using 31334-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 31334-1-AP (EPN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 31334-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 31334-1-AP (EPN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

EPN2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 31334-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

KIAA1065, Epsin-2, epsin 2, EPS-15-interacting protein 2, EPS 15 interacting protein 2

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Tested Applications

• Positive WB detected in: HEK-293T cells, HepG2 cells, SH-SY5Y cells
• Positive IHC detected in: mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31334-1-AP targets EPN2 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EPN2 fusion protein Ag35194
• Full Name: epsin 2
• Calculated Molecular Weight: 584 aa, 62 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC093972
• Gene Symbol: EPN2
• Gene ID (NCBI): 22905
• RRID: AB_3669948
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: O95208
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EPN2 (EPS 15 interacting protein 2) is a protein that interacts with clathrin and adaptor-related protein complex 2, alpha 1 subunit. EPN2 is found in a brain-derived clathrin-coated vesicle fraction and localizes to the peri-Golgi region and the cell periphery.