Tested Applications
Positive WB detected in | A431 cells, HEK-293 cells, HeLa cells, K-562 cells |
Positive IF/ICC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:5000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
14469-1-AP targets EPN1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | EPN1 fusion protein Ag5838 Predict reactive species |
Full Name | epsin 1 |
Calculated Molecular Weight | 60 kDa |
Observed Molecular Weight | 65 kDa |
GenBank Accession Number | BC044651 |
Gene Symbol | EPN1 |
Gene ID (NCBI) | 29924 |
RRID | AB_3085436 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9Y6I3 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Epsin 1 (EPN1) belongs to the epsin family, which also includes EPN2 and EPN3. They belong to the endocytic clathrin family and are involved in receptor endocytosis. EPN1 is widely and highly expressed in tumors.
Protocols
Product Specific Protocols | |
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WB protocol for EPN1 antibody 14469-1-AP | Download protocol |
IF protocol for EPN1 antibody 14469-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |