EPN1 Polyclonal antibody

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  WB analysis using 14469-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14469-1-AP (EPN1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 14469-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EPN1 antibody (14469-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

EPN1 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 14469-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Epsin-1, EPS-15-interacting protein 1, EH domain-binding mitotic phosphoprotein

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Tested Applications

• Positive WB detected in: A431 cells, HEK-293 cells, HeLa cells, K-562 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14469-1-AP targets EPN1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EPN1 fusion protein Ag5838
• Full Name: epsin 1
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC044651
• Gene Symbol: EPN1
• Gene ID (NCBI): 29924
• RRID: AB_3085436
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y6I3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Epsin 1 (EPN1) belongs to the epsin family, which also includes EPN2 and EPN3. They belong to the endocytic clathrin family and are involved in receptor endocytosis. EPN1 is widely and highly expressed in tumors.