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  • Featured Product
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EPHX1 Monoclonal antibody, PBS Only

EPHX1 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B6A2

Cat no : 66982-1-PBS

Synonyms

EPHX, EPHX1, EPOX, Epoxide hydratase, Epoxide hydrolase 1, MEH, Microsomal epoxide hydrolase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EPHX1 Monoclonal antibody (66982-1-Ig)

    WB analysis using 66982-1-Ig (same clone as 66982-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66982-1-Ig (EPHX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66982-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using EPHX1 Monoclonal antibody (66982-1-Ig)

    WB analysis of HeLa using 66982-1-Ig (same clone as 66982-1-PBS)

    WB result of EPHX1 antibody (66982-1-Ig; 1:120000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPHX1 transfected HeLa cells. This data was developed using the same antibody clone with 66982-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using EPHX1 Monoclonal antibody (66982-1-Ig)

    WB analysis of A549 using 66982-1-Ig (same clone as 66982-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 66982-1-Ig (EPHX1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66982-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of 4T1 cells using EPHX1 Monoclonal antibody (66982-1-Ig)

    WB analysis of 4T1 using 66982-1-Ig (same clone as 66982-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 66982-1-Ig (EPHX1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66982-1-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66982-1-PBS targets EPHX1 in WB, Indirect ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen EPHX1 fusion protein Ag25820
Full Name epoxide hydrolase 1, microsomal (xenobiotic)
Calculated Molecular Weight 53 kDa
Observed Molecular Weight53 kDa
GenBank Accession NumberBC003567
Gene Symbol EPHX1
Gene ID (NCBI) 2052
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

EPHX1, also known as EPHX and EPOX, belongs to the peptidase S33 family. EPHX1 is an evolutionarily highly conserved biotransformation enzyme expressed in nearly all tissues and localized mainly in the microsomal fraction of the endoplasmic reticulum of eukaryotic cells. EPHX1 plays a role in the metabolism of endogenous lipids such as epoxide-containing fatty acids. The calculated molecular weight of EPHX1 is 53 kDa (PMID: 26216302).