EPHB4 Polyclonal antibody

• Featured Product
• KD/KO Validated

EPHB4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

22

Reviews (1)

Cat no : 20883-1-AP

Synonyms

MYK1, HTK, Ephrin type-B receptor 4, Ephrin type B receptor 4, EPH receptor B4

---

Validation Data Gallery

• 

  WB analysis using 20883-1-AP

  HT-29 cells were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 20883-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20883-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 20883-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 20883-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 20883-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of DU 145 using 20883-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of DU 145 using 20883-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 20883-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 20883-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 20883-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20883-1-AP (EPHB4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 20883-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20883-1-AP (EPHB4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 20883-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 20883-1-AP (EPHB4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 20883-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 20883-1-AP (EPHB4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 20883-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 20883-1-AP (EPHB4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 20883-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 20883-1-AP (EPHB4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 20883-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 20883-1-AP (EPHB4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 20883-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 20883-1-AP (EPHB4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HT-29 using 20883-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using EPHB4 antibody (20883-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: HT-29 cells, rat testis tissue, HeLa cells, HT-1080 cells, PC-3 cells, DU 145 cells
• Positive IHC detected in: human lung cancer tissue, human pancreas tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HT-29 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IHC: See 11 publications below
• IF: See 7 publications below
• FC: See 1 publications below

Product Information

20883-1-AP targets EPHB4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EPHB4 fusion protein Ag10033
• Full Name: EPH receptor B4
• Calculated Molecular Weight: 987 aa, 108 kDa
• Observed Molecular Weight: 110-120 kDa
• GenBank Accession Number: BC052804
• Gene Symbol: EPHB4
• Gene ID (NCBI): 2050
• RRID: AB_10897158
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IF	Small; Specific Photothermal Ablation Therapy of Endometriosis by Targeting Delivery of Gold Nanospheres.; Authors - Xiaomeng Guo
human	IF	Drug Deliv; Near infrared light mediated photochemotherapy for efficiently treating deep orthotopic tumors guided by ultrasound imaging.; Authors - Zuhua Wang
	WB,IHC	J Cell Physiol; Intermittent parathyroid hormone promotes cementogenesis via ephrinB2-EPHB4 forward signaling.; Authors - Tiancheng Li
human	WB	J Cell Mol Med; Kinome multigenic panel identified novel druggable EPHB4-V871I somatic variant in high-risk neuroblastoma.; Authors - Immacolata Andolfo
human	FC	Phytomedicine; Sanguinarine combats hypoxia-induced activation of EphB4 and HIF-1α pathways in breast cancer.; Authors - Qi Su
rat	IHC	Commun Biol; The application of tissue-engineered fish swim bladder vascular graft.; Authors - Hualong Bai

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Marsilius (Verified Customer) (04-13-2021)

This antibody is useful for staining.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: brain

EPHB4 Antibody Immunofluorescence validation (1:100 dilution) in brain (Cat no:20883-1-AP)

Related Products

ERK1/2 Polyclonal antibody

16443-1-AP

EPHA1-specific Polyclonal antibody

18698-1-AP

EphA4 Polyclonal antibody

21875-1-AP

HOXA9 Polyclonal antibody

18501-1-AP