EPHB1 Monoclonal antibody, PBS Only (Detector)

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  WB analysis of mouse cerebellum using 67080-1-Ig (same clone as 67080-1-PBS)

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 67080-1-Ig (EPHB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67080-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 67080-1-Ig (same clone as 67080-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67080-1-Ig (EPHB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67080-1-PBS in a different storage buffer formulation.

• 

  WB analysis of fetal human brain using 67080-1-Ig (same clone as 67080-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 67080-1-Ig (EPHB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67080-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50740-1

  Cytometric bead array standard curve of MP50740-1, EPHB1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67080-2-PBS. Detection antibody: 67080-1-PBS. Standard:Ag16347. Range: 0.195-200 ng/mL.

EPHB1 Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA

Cat No. 67080-1-PBS

Clone No.1B7C12

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, Cytometric bead array, Indirect ELISA

Synonyms

EPH receptor B1, EPH like kinase 6, ELK, EK6, EC:2.7.10.1

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Product Information

67080-1-PBS targets EPHB1 as part of a matched antibody pair:

MP50740-1: 67080-2-PBS capture and 67080-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: EPHB1 fusion protein Ag16347
• Full Name: EPH receptor B1
• Calculated Molecular Weight: 984 aa, 110 kDa
• Observed Molecular Weight: 110-120 kDa
• GenBank Accession Number: BC111744
• Gene Symbol: EPHB1
• Gene ID (NCBI): 2047
• RRID: AB_2882388
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P54762
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

EPHB1, also named as EPHT2, NET, HEK6 and ELK, belongs to the protein kinase superfamily, Tyr protein kinase family, and Ephrin receptor subfamily. It is a receptor for members of the ephrin-B family. EPHB1 binds to ephrin-B1, -B2 and -B3. EPHB1 may be involved in cell-cell interactions in the nervous system. EPHB1 catalyzes the reaction: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.