EPHA2 Monoclonal antibody, PBS Only

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  WB analysis using 66736-1-Ig (same clone as 66736-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66736-1-Ig (EPHA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66736-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66736-1-Ig (same clone as 66736-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EPHA2 antibody (66736-1-Ig, Clone: 1A9C3 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66736-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

EPHA2 Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 66736-1-PBS

Clone No.1A9C3

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Ephrin type-A receptor 2, Ephrin type A receptor 2, EPH receptor A2, ECK, EC:2.7.10.1

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Product Information

66736-1-PBS targets EPHA2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: EPHA2 fusion protein Ag22566
• Full Name: EPH receptor A2
• Calculated Molecular Weight: 976 aa, 108 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC037166
• Gene Symbol: EPHA2
• Gene ID (NCBI): 1969
• RRID: AB_2882086
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P29317
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

EPHA2 (Ephrin type-A receptor 2) belongs to the receptor tyrosine kinase (RTK) family, with 16 known receptors and 9 known membrane-bound ligands in all species. Based on the extracellular domain sequence homology, structure, and binding affinity, Eph receptors and their ephrin ligands can be divided into A and B subtypes (PMID: 33962882). EPHA2 contains a conserved N-terminal ligand-bound extracellular domain, a transmembrane domain, and a conserved tyrosine kinase domain (PMID: 8070404).