EPHA2 Polyclonal antibody

EPHA2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 25749-1-AP

Synonyms

Epithelial cell kinase, Ephrin type-A receptor 2, Ephrin type A receptor 2, EPH receptor A2, ECK

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Validation Data Gallery

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  WB analysis using 25749-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25749-1-AP (EPHA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A375 cells, A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

25749-1-AP targets EPHA2 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EPHA2 fusion protein Ag22661
• Full Name: EPH receptor A2
• Calculated Molecular Weight: 976 aa, 108 kDa
• Observed Molecular Weight: 125-130 kDa
• GenBank Accession Number: BC037166
• Gene Symbol: EPHA2
• Gene ID (NCBI): 1969
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EPHA2 (Ephrin type-A receptor 2) belongs to the receptor tyrosine kinase (RTK) family, with 16 known receptors and 9 known membrane-bound ligands in all species. Based on the extracellular domain sequence homology, structure, and binding affinity, Eph receptors and their ephrin ligands can be divided into A and B subtypes (PMID: 33962882). EPHA2 contains a conserved N-terminal ligand-bound extracellular domain, a transmembrane domain, and a conserved tyrosine kinase domain (PMID: 8070404).

Publications

Species	Application	Title
human	WB	Anticancer Res; High αv Integrin Level of Cancer Cells Is Associated with Development of Brain Metastasis in Athymic Rats.; Authors - Yingjen Jeffrey Wu