EPB41L4B Recombinant antibody, PBS Only (Capture)

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  WB analysis using 84732-2-RR (same clone as 84732-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84732-2-RR (EPB41L4B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84732-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01525-2

  Cytometric bead array standard curve of MP01525-2, EPB41L4B Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84732-2-PBS. Detection antibody: 84732-3-PBS. Standard: Ag36353. Range: 0.781-100 ng/mL.

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  Cytometric bead array standard curve of MP01525-1

  Cytometric bead array standard curve of MP01525-1, EPB41L4B Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84732-2-PBS. Detection antibody: 84732-1-PBS. Standard: Ag36353. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 84732-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84732-2-RR against Human EPB41L4B were performed. The affinity constant is 6.86 nM.

EPB41L4B {ptg:Brand} Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Cat No. 84732-2-PBS

Clone No.242186G4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Cytometric bead array, Indirect ELISA

Synonyms

242186G4, Band 4.1 like protein 4B, CG1, EHM2, FERM containing protein CG1

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Product Information

84732-2-PBS targets EPB41L4B as part of a matched antibody pair:

MP01525-1: 84732-2-PBS capture and 84732-1-PBS detection (validated in Cytometric bead array)

MP01525-2: 84732-2-PBS capture and 84732-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: EPB41L4B fusion protein Ag36353
• Full Name: erythrocyte membrane protein band 4.1 like 4B
• Calculated Molecular Weight: 99kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: NM_00138562
• Gene Symbol: EPB41L4B
• Gene ID (NCBI): 54566
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9H329
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

The 4.1 proteins, encoded by the EPB41 (erythrocyte protein band 4.1) genes, are components of the cortical cytoskeleton underlying the cell membrane. The family of 4.1 proteins consists of the eponymous 4.1R protein first identified in erythrocytes (gene: EPB41), 4.1N (EPB41L1), 4.1G (EPB41L2), 4.1B (EPB41L3) as well as the less closely related members NBL4 (EPB41L4A), EHM2 (EPB41L4B) and EPB41L5 (EPB41L5). EHM2 is conspicuous in tumor cells with high migratory potential, such as metastatic melanoma and fibrosarcoma cells. In prostate cancer, EHM2 has been reported to be overexpressed and to diminish adhesion of prostate cancer cells to collagen. (PMID: 20860828)