EPB41L4B Polyclonal antibody

• 

  WB analysis using 31669-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31669-1-AP (EPB41L4B antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

EPB41L4B Polyclonal Antibody for WB, ELISA

Cat No. 31669-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

Band 4.1 like protein 4B, CG1, DKFZp761N1814, EHM2, EPB41L4B, FERM containing protein CG1, FLJ21596, Protein EHM2

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Tested Applications

• Positive WB detected in: Caco-2 cells, HEK-293T cells, MCF-7 cells, U-87 MG cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31669-1-AP targets EPB41L4B in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EPB41L4B fusion protein Ag36353
• Full Name: erythrocyte membrane protein band 4.1 like 4B
• Calculated Molecular Weight: 99kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: NM_00138562
• Gene Symbol: EPB41L4B
• Gene ID (NCBI): 54566
• RRID: AB_3670069
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9H329
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The 4.1 proteins, encoded by the EPB41 (erythrocyte protein band 4.1) genes, are components of the cortical cytoskeleton underlying the cell membrane. The family of 4.1 proteins consists of the eponymous 4.1R protein first identified in erythrocytes (gene: EPB41), 4.1N (EPB41L1), 4.1G (EPB41L2), 4.1B (EPB41L3) as well as the less closely related members NBL4 (EPB41L4A), EHM2 (EPB41L4B) and EPB41L5 (EPB41L5). EHM2 is conspicuous in tumor cells with high migratory potential, such as metastatic melanoma and fibrosarcoma cells. In prostate cancer, EHM2 has been reported to be overexpressed and to diminish adhesion of prostate cancer cells to collagen. (PMID: 20860828)