HeLa cells were subjected to SDS PAGE followed by western blot with 15437-1-AP (EPB41L2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
HeLa cells were subjected to SDS PAGE followed by western blot with 15437-1-AP (EPB41L2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 15437-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 15437-1-AP (EPB41L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Jurkat cells were subjected to SDS PAGE followed by western blot with 15437-1-AP (EPB41L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 15437-1-AP
IP result of anti-EPB41L2 (IP:15437-1-AP, 4ug; Detection:15437-1-AP 1:4000) with HEK-293 cells lysate 1610 ug.
IP result of anti-EPB41L2 (IP:15437-1-AP, 4ug; Detection:15437-1-AP 1:4000) with HEK-293 cells lysate 1610 ug.
IHC staining of human ovary tumor using 15437-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15437-1-AP (EPB41L2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15437-1-AP (EPB41L2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 15437-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15437-1-AP (EPB41L2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15437-1-AP (EPB41L2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of A431 using 15437-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using EPB41L2 antibody (15437-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using EPB41L2 antibody (15437-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
IF Staining of HeLa using 15437-1-AP
Immunofluorescent analysis of HeLa cells using 15437-1-AP (EPB41L2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of HeLa cells using 15437-1-AP (EPB41L2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
A431 cells, HeLa cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:1000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
15437-1-AP targets EPB41L2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
EPB41L2, also known as 4.1G, is a member of the protein 4.1 family, which generally serves as adaptors linking transmembrane proteins to the cytoskeleton(PMID: 21482674).
HeLa cells were subjected to SDS PAGE followed by western blot with 15437-1-AP (EPB41L2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 15437-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 15437-1-AP (EPB41L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human ovary tumor using 15437-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15437-1-AP (EPB41L2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 15437-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15437-1-AP (EPB41L2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of HEK-293 using 15437-1-AP
IP result of anti-EPB41L2 (IP:15437-1-AP, 4ug; Detection:15437-1-AP 1:4000) with HEK-293 cells lysate 1610 ug.
IF/ICC Figures
IF Staining of A431 using 15437-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using EPB41L2 antibody (15437-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
IF Staining of HeLa using 15437-1-AP
Immunofluorescent analysis of HeLa cells using 15437-1-AP (EPB41L2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
EPB41L2 Polyclonal antibody
Catalog Number
15437-1-AP
Citations
-
Dilutions
WB : 1:500-1:1000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:250-1:1000 IF/ICC : 1:50-1:500
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
