HIF2α/EPAS1 Polyclonal antibody

HIF2α/EPAS1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

17

Reviews (4)

Cat no : 26422-1-AP

Synonyms

bHLHe73, ECYT4, EPAS 1, EPAS1, HIF 1 alpha like factor, HIF 2 alpha, HIF2 alpha, HIF2A, HIF-2A, HIF2α, HIF-2α, HLF, Member of PAS protein 2, MOP2, PASD2

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Validation Data Gallery

• 

  WB analysis using 26422-1-AP

  Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with 26422-1-AP (EPAS1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Cobalt Chloride treated HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 15 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Product Information

26422-1-AP targets HIF2α/EPAS1 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HIF2α/EPAS1 fusion protein Ag24886
• Full Name: endothelial PAS domain protein 1
• Calculated Molecular Weight: 96 kDa
• Observed Molecular Weight: 100-120 kDa
• GenBank Accession Number: BC051338
• Gene Symbol: HIF2α
• Gene ID (NCBI): 2034
• RRID: AB_2880510
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HIF2A, also named as EPAS1, is a 870 amino acid protein, which is expressed in most tissues, with highest levels in placenta, lung and heart. HIF2A colocalizes with HIF3A in the nucleus and speckles. HIF2A as a transcription factor involves in the induction of oxygen regulated genes. HIF2A binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. HIF2A regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. HIF2A may also play a role in the formation of the endothelium that gives rise to the blood brain barrier. The calculated molecular weight of HIF2A is 96 kDa, but In normoxia, HIF2A is probably hydroxylated on Pro-405 and Pro-531 by EGLN1/PHD1, EGLN2/PHD2 and/or EGLN3/PHD3. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization. The modified HIf2A is about 100-120 kDa.

Publications

Species	Application	Title
human	WB	Cell Rep; CGG repeats in the human FMR1 gene regulate mRNA localization and cellular stress in developing neurons; Authors - Carissa L Sirois
	WB	Cell Mol Life Sci; Iron increases lipid deposition via oxidative stress-mediated mitochondrial dysfunction and the HIF1α-PPARγ pathway.; Authors - Chang-Chun Song
human	WB	Chemosphere; Organophosphate flame retardant TDCPP: A risk factor for renal cancer?; Authors - Xuan Zhou
human	WB,IF	Theranostics; Acidic Microenvironment Up-Regulates Exosomal miR-21 and miR-10b in Early-Stage Hepatocellular Carcinoma to Promote Cancer Cell Proliferation and Metastasis.; Authors - Xiao-Peng Tian
human	WB	Proc Natl Acad Sci U S A; Induction of the Mdm2 gene and protein by kinase signaling pathways is repressed by the pVHL tumor suppressor; Authors - Alexander R Mabry
human	WB,IHC,CoIP	Cancer Biol Med; MIIP inhibits clear cell renal cell carcinoma proliferation and angiogenesis via negative modulation of the HIF-2α-CYR61 axis; Authors - Fengqi Yan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jingwen (Verified Customer) (01-27-2020)

It worked well with cancer tissue when I performed immunostaining.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Cancer tissue

FH

LUNFENG (Verified Customer) (01-27-2020)

NOT VERY GOOD

• Applications: Immunofluorescence,

FH

Jie (Verified Customer) (01-27-2020)

Positive staining in heart tissue, however, no difference were observed between KO mice and WT mice

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: mouse heart

FH

CHIHMIN (Verified Customer) (02-01-2019)

It works well in Western.

• Applications: Western Blot,