ENTPD3 Polyclonal antibody

ENTPD3 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 13021-1-AP

Synonyms

Ecto-ATPase 3, Ecto-ATP diphosphohydrolase 3, Ecto-apyrase 3, EC:3.6.1.5, CD39L3

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Validation Data Gallery

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  WB analysis of human brain using 13021-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13021-1-AP (ENTPD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 13021-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13021-1-AP (ENTPD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 13021-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 13021-1-AP (ENTPD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of U2OS using 13021-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using ENTPD3 antibody (13021-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human brain tissue, Jurkat cells, Raji cells
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

13021-1-AP targets ENTPD3 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ENTPD3 fusion protein Ag4114
• Full Name: ectonucleoside triphosphate diphosphohydrolase 3
• Calculated Molecular Weight: 529 aa, 59 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC029869
• Gene Symbol: ENTPD3
• Gene ID (NCBI): 956
• RRID: AB_2100082
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ENTPD3, also known as NTPDase3 or CD39L3, belongs to the GDA1/CD39 NTPase family. ENTPD3 has a threefold preference for the hydrolysis of ATP over ADP. ENTPD3 is expressed in adult brain, pancreas, spleen and prostate, but not in liver and peripheral blood leukocytes. ENTPD3 has 2 isoforms with the molecular mass of 51 and 59 kDa.

Publications

Species	Application	Title
mouse	WB	Nat Commun; Impaired calcium signaling in astrocytes modulates autism spectrum disorder-like behaviors in mice.; Authors - Qian Wang