CD39/ENTPD1 Polyclonal antibody

CD39/ENTPD1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 14211-1-AP

Synonyms

ENTPD1, Ecto apyrase, EC:3.6.1.5, CD39, ATP-DPH



Tested Applications

Positive WB detected inmouse placenta tissue, human placenta tissue
Positive IHC detected inhuman placenta tissue, human tonsillitis tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman placenta tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14211-1-AP targets CD39/ENTPD1 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD39/ENTPD1 fusion protein Ag5429
Full Name ectonucleoside triphosphate diphosphohydrolase 1
Calculated Molecular Weight 58 kDa
Observed Molecular Weight 50 kDa,70-100 kDa
GenBank Accession NumberBC047664
Gene Symbol CD39
Gene ID (NCBI) 953
RRIDAB_10638609
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ENTPD1(Ectonucleoside triphosphate diphosphohydrolase 1) is also named CD39, NTPDase 1, and Ecto-ATPDase 1 and belongs to the GDA1/CD39 NTPase family. In the nervous system, it can hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. It is a noncovalent tetrameric protein and detergent inhibition of enzymatic activity is caused by dissociation of ectoapyrase tetramers into monomers(PMID: 9733785 ). And it is a 70- to 100-kDa protein that is heavily N-glycosylated(PMID:7930580). This protein has 6 glycosylation sites and 3 isoforms(58 kDa, 59 kDa, and 34 kDa) produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for CD39/ENTPD1 antibody 14211-1-APDownload protocol
IHC protocol for CD39/ENTPD1 antibody 14211-1-APDownload protocol
IF protocol for CD39/ENTPD1 antibody 14211-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IF

Int J Biol Sci

CD73/NT5E-mediated ubiquitination of AURKA regulates alcohol-related liver fibrosis via modulating hepatic stellate cell senescence

Authors - Zhenni Liu
humanIF

Genomics Proteomics Bioinformatics

Single-cell Immune Landscape of Human Recurrent Miscarriage.

Authors - Feiyang Wang
humanIHC

Oncotarget

Expression of CD38 in myeloma bone niche: A rational basis for the use of anti-CD38 immunotherapy to inhibit osteoclast formation.

Authors - Federica Costa
ratIHC

Am J Physiol Gastrointest Liver Physiol

Water intake accelerates ATP release from myofibroblast cells in rats: ATP-mediated podoplanin-dependent control for physiological function and immunity.

Authors - Moyuru Hayashi
humanWB

BMC Med Genomics

Identification of differential gene expression profile from peripheral blood cells of military pilots with hypertension by RNA sequencing analysis.

Authors - Xing-Cheng Zhao
ratWB

J Gerontol A Biol Sci Med Sci

Hypoxanthine Induces Signs of Bladder Aging with Voiding dysfunction and Lower Urinary Tract Remodeling

Authors - Lori A Birder