ENT2 Polyclonal antibody

ENT2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 26082-1-AP

Synonyms

SLC29A2, Nucleoside transporter, ei-type, hENT2, Equilibrative nitrobenzylmercaptopurine riboside-insensitive nucleoside transporter, Equilibrative NBMPR-insensitive nucleoside transporter

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Validation Data Gallery

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  WB analysis using 26082-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26082-1-AP (ENT2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26082-1-AP targets ENT2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ENT2 fusion protein Ag23310
• Full Name: solute carrier family 29 (nucleoside transporters), member 2
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC093634
• Gene Symbol: SLC29A2
• Gene ID (NCBI): 3177
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC29A2 also known as ENT2, comprises 456 amino acids and shares 46% amino acid identity with ENT1. ENT2 is involved in the facilitative transport of nucleosides and nucleobases and maintains their cellular homeostasis. The predicted molecular weight of ENT1 is 50 kDa, while 45 kDa band may be observed due to the deglycosylation (PMID: 10722669)