ENPP2 Monoclonal antibody

ENPP2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2A10D9

Cat no : 68724-1-Ig

Synonyms

AUTOTAXIN, E-NPP 2, EC:3.1.4.39, E NPP 2, ATX X

---

Validation Data Gallery

• 

  WB analysis of HuH-7 using 68724-1-Ig

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 68724-1-Ig (ENPP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-87 MG using 68724-1-Ig

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 68724-1-Ig (ENPP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68724-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68724-1-Ig (ENPP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: pig brain tissue, HuH-7 cells, U-87 MG cells, rabbit brain tissue, rat brain tissue, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68724-1-Ig targets ENPP2 in WB, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: ENPP2 fusion protein Ag5499
• Full Name: ectonucleotide pyrophosphatase/phosphodiesterase 2
• Calculated Molecular Weight: 99 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: BC034961
• Gene Symbol: ENPP2
• Gene ID (NCBI): 5168
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ENPP2, Autotaxin or Extracellular lysophospholipase D, is a 863 amino acid secreted protein, which is detected in blood plasma (at protein level) (PubMed:12176993, PubMed:26371182). ENPP2 is Predominantly expressed in brain, placenta, ovary, and small intestine. ENPP2 is expressed in a number of carcinomas such as hepatocellular and prostate carcinoma, neuroblastoma and non-small-cell lung cancer. ENPP2 is expressed in body fluids such as plasma, cerebral spinal fluid (CSF), saliva, follicular and amniotic fluids. The calculated molecular weight of ENPP2 is about 100 kDa (PMID: 22301782), but modified ENPP2 is 120 kDa (PMID: 19808661). ENPP2 Hydrolyzes lysophospholipids to produce the signaling molecule lysophosphatidic acid (LPA) in extracellular fluids (PubMed:15769751, PubMed:26371182, PubMed:27754931).