ENPP2 Polyclonal antibody

ENPP2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

9

Reviews (1)

Cat no : 14243-1-AP

Synonyms

ATX, ATX X, Autotaxin, E NPP 2, ENPP2, LysoPLD, PD IALPHA, PDNP2

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Validation Data Gallery

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  WB analysis using 14243-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14243-1-AP (Autotaxin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 14243-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14243-1-AP (Autotaxin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 14243-1-AP

  IP result of anti-Autotaxin (IP:14243-1-AP, 5ug; Detection:14243-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human liver cancer using 14243-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14243-1-AP (Autotaxin antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human small intestine using 14243-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 14243-1-AP (Autotaxin antibody at dilution of 1:50.

• 

  IHC staining of human small intestine using 14243-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 14243-1-AP (Autotaxin antibody at dilution of 1:50.

• 

  IHC staining of human liver cancer using 14243-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14243-1-AP (Autotaxin antibody at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, human placenta tissue, mouse kidney tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human liver cancer tissue, human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 3 publications below

Product Information

14243-1-AP targets ENPP2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ENPP2 fusion protein Ag5499
• Full Name: ectonucleotide pyrophosphatase/phosphodiesterase 2
• Calculated Molecular Weight: 99 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: BC034961
• Gene Symbol: ENPP2
• Gene ID (NCBI): 5168
• RRID: AB_2878033
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ENPP2, Autotaxin or Extracellular lysophospholipase D, is a 863 amino acid secreted protein, which is detected in blood plasma (at protein level) (PubMed:12176993, PubMed:26371182). ENPP2 is Predominantly expressed in brain, placenta, ovary, and small intestine. ENPP2 is expressed in a number of carcinomas such as hepatocellular and prostate carcinoma, neuroblastoma and non-small-cell lung cancer. ENPP2 is expressed in body fluids such as plasma, cerebral spinal fluid (CSF), saliva, follicular and amniotic fluids. The calculated molecular weight of ENPP2 is about 100 kDa (PMID: 22301782), but modified ENPP2 is 120 kDa (PMID: 19808661). ENPP2 Hydrolyzes lysophospholipids to produce the signaling molecule lysophosphatidic acid (LPA) in extracellular fluids (PubMed:15769751, PubMed:26371182, PubMed:27754931).

Publications

Species	Application	Title
human	WB	PLoS Pathog; Reduced IRF4 expression promotes lytic phenotype in Type 2 EBV-infected B cells.; Authors - Jillian A Bristol
human,mouse	WB	Cells; Role of Aberrantly Activated Lysophosphatidic Acid Receptor 1 Signaling Mediated Inflammation in Renal Aging.; Authors - Yongjie Jin
mouse	WB	Front Pharmacol; UPLC/Q-TOFMS-Based Metabolomics Approach to Reveal the Protective Role of Other Herbs in An-Gong-Niu-Huang Wan Against the Hepatorenal Toxicity of Cinnabar and Realgar.; Authors - Fangbo Xia
human	IHC	Heliyon; Secreted proteins in plasma and placenta as novel non-invasive biomarkers for intrahepatic cholestasis of pregnancy: A case-control study; Authors - Lingyan Chen
mouse	WB	Am J Physiol Cell Physiol; The Ecto-Nucleotide Pyrophosphatase/Phosphodiesterase 2 Promotes Early Osteoblast Differentiation and Mineralization in Stromal Stem Cells; Authors - Irina L Tourkova
mouse	WB	Thyroid; Increased thyroid hormone action alleviates hippocampal damage by down-regulating neuronal type-Ⅰ interferon signaling/necroptosis in diabetes-associated cognitive dysfunction; Authors - Ling Tian

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jacqueline (Verified Customer) (05-14-2019)

Works perfectly in western blot using concentrated media of cells that are cultured in serum-depleted conditions. Serum is a good positive control or secreted ENPP2/ATX. In our hands ~120kD

• Applications: Western Blot, Immunoprecipitation, Cell culture,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Media from PDAC cells and fibroblasts

ENPP2 Antibody Western Blot,Immunoprecipitation,Cell culture, validation (1:500 dilution) in Media from PDAC cells and fibroblasts (Cat no:14243-1-AP)