ENOSF1 Recombinant antibody, PBS Only (Detector)

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  WB analysis using 83769-2-RR (same clone as 83769-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83769-2-RR (ENOSF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83769-2-PBS in a different storage buffer formulation.

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  FC experiment of Hela using 83769-2-RR (same clone as 83769-2-PBS)

  1x10^6 Hela cells were intracellularly stained with 0.25 ug ENOSF1 Recombinant antibody (83769-2-RR, Clone:240796D2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83769-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00758-1

  Cytometric bead array standard curve of MP00758-1, ENOSF1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83769-1-PBS. Detection antibody: 83769-2-PBS. Standard: Ag34665. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 83769-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83769-2-RR against Human ENOSF1 were performed. The affinity constant is below 1 pM.

ENOSF1 {ptg:Brand} Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Cat No. 83769-2-PBS

Clone No.240796D2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

TYMSAS, rTS, L-fuconate dehydratase, enolase superfamily member 1, EC:4.2.1.68

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Product Information

83769-2-PBS targets ENOSF1 as part of a matched antibody pair:

MP00758-1: 83769-1-PBS capture and 83769-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ENOSF1 fusion protein Ag34665
• Full Name: enolase superfamily member 1
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC001285
• Gene Symbol: ENOSF1
• Gene ID (NCBI): 55556
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q7L5Y1
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

ENOSF1 (Mitochondrial enolase superfamily member 1) is also named as RTS and TYMSAS. The ENOSF1 gene, responsible for encoding the protein mitochondrial enolase superfamily member 1 (ENOF1), exhibits three distinct isoforms. One of these isoforms has been identified as an l-fuconate dehydratase, actively participating in the catabolism of L-fucose, a sugar integral to the carbohydrate composition of cellular glycoproteins (PMID: 38203276). The ENOSF1 transcript has been shown to act as antisense RNA and inhibit TYMS expression (PMID: 35931051).