ENOSF1 Polyclonal antibody

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  WB analysis using 31022-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31022-1-AP (ENOSF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 31022-1-AP

  IP result of anti-ENOSF1 (IP:31022-1-AP, 4ug; Detection:31022-1-AP 1:2000) with MCF-7 cells lysate 1600 ug.

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  FC experiment of HeLa using 31022-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug ENOSF1 Polyclonal antibody (31022-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

ENOSF1 Polyclonal Antibody for WB, FC (Intra), IP, ELISA

Cat No. 31022-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), IP, ELISA

Synonyms

EC:4.2.1.68, enolase superfamily member 1, L-fuconate dehydratase, rTS, TYMSAS

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Tested Applications

• Positive WB detected in: HeLa cells, K-562 cells, MCF-7 cells
• Positive IP detected in: MCF-7 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31022-1-AP targets ENOSF1 in WB, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ENOSF1 fusion protein Ag34665
• Full Name: enolase superfamily member 1
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC001285
• Gene Symbol: ENOSF1
• Gene ID (NCBI): 55556
• RRID: AB_3669818
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q7L5Y1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ENOSF1 (Mitochondrial enolase superfamily member 1) is also named as RTS and TYMSAS. The ENOSF1 gene, responsible for encoding the protein mitochondrial enolase superfamily member 1 (ENOF1), exhibits three distinct isoforms. One of these isoforms has been identified as an l-fuconate dehydratase, actively participating in the catabolism of L-fucose, a sugar integral to the carbohydrate composition of cellular glycoproteins (PMID: 38203276). The ENOSF1 transcript has been shown to act as antisense RNA and inhibit TYMS expression (PMID: 35931051).