ENOPH1 Polyclonal antibody

ENOPH1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 17731-1-AP

Synonyms

MASA, MST145, Enolase-phosphatase E1, EC:3.1.3.77, E1

---

Validation Data Gallery

• 

  WB analysis using 17731-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17731-1-AP (ENOPH1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 17731-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17731-1-AP (ENOPH1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 17731-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17731-1-AP (ENOPH1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 17731-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17731-1-AP (ENOPH1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 17731-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17731-1-AP (ENOPH1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of U-251 using 17731-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U-251 cells using ENOPH1 antibody (17731-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: HL-60 cells, K-562 cells, L02 cells, mouse liver tissue, rat liver tissue
• Positive IHC detected in: human liver cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U-251 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

17731-1-AP targets ENOPH1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ENOPH1 fusion protein Ag12131
• Full Name: enolase-phosphatase 1
• Calculated Molecular Weight: 261 aa, 29 kDa
• Observed Molecular Weight: 29-35 kDa
• GenBank Accession Number: BC065815
• Gene Symbol: ENOPH1
• Gene ID (NCBI): 58478
• RRID: AB_2099471
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ENOPH1, also named as MASA and MSTP145, belongs to the HAD-like hydrolase superfamily and MasA/MtnC family. ENOPH1 is a newly identified enzyme involved in L-methionine biosynthesis, which is associated with anxiety and depression. Studies have shown that ENOPH1 plays a crucial role in promoting the proliferation and migration of glioma cells (PMID: 32654229). ENOPH1 has 2 isoforms with the molecular mass of 13 and 29 kDa.

Publications

Species	Application	Title
mouse	WB	Exp Neurol; Evidence that enolase-phosphatase 1 exacerbates early cerebral ischemia injury and blood-brain barrier breakdown by enhancing extracellular matrix destruction and inhibiting the interaction between ADI1 and MT1-MMP; Authors - Dexin Yang