NSE/ENO2-specific Polyclonal antibody

• Featured Product
• KD/KO Validated

NSE/ENO2-specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 55235-1-AP

Synonyms

Enolase 2, enolase 2 (gamma, neuronal), Gamma enolase, Neural enolase, Neuron specific enolase, NSE, NSE/ENO2-specific

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Validation Data Gallery

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  WB analysis of U-251 using 55235-1-AP

  WB result of NSE/ENO2-specific antibody (55235-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NSE/ENO2-specific transfected U-251 cells.

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  WB analysis of SH-SY5Y using 55235-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 55235-1-AP (NSE/ENO2-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 55235-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55235-1-AP (NSE/ENO2-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 55235-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55235-1-AP (NSE/ENO2-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 55235-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55235-1-AP (NSE/ENO2-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 55235-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 55235-1-AP (NSE/ENO2-specific antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 55235-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 55235-1-AP (NSE/ENO2-specific antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human stomach cancer using 55235-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55235-1-AP (NSE/ENO2-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 55235-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55235-1-AP (NSE/ENO2-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 55235-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55235-1-AP (NSE/ENO2-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 55235-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55235-1-AP (NSE/ENO2-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, mouse brain tissue, HEK-293 cells, HepG2 cells, U-251 cells
• Positive IHC detected in: human brain tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

55235-1-AP targets NSE/ENO2-specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: enolase 2 (gamma, neuronal)
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: NM_001975
• Gene Symbol: NSE
• Gene ID (NCBI): 2026
• RRID: AB_10863290
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NSE, also named as ENO2, belongs to the enolase family. Enolases are cytoplasmic glycolytic enzymes that may be involved in differentiation. The enolase has three isoenzymes, alpha, beta and gamma. The alpha form is expressed in most tissues, whereas the beta form is expressed in muscle tissue. The gamma enolase (ENO2), a homodimer, is primarily localized in neurons and neuroendocrine cells and is a cancer diagnostic marker for brain tumors (PMID:7520111). ENO2 plays a role in the glycolysis-related energy pathway and might be involved in higher metabolic activity during the day than at night, at least in part. This antibody is specific to ENO2 and has no cross reaction to ENO1 and ENO3.

Publications

Species	Application	Title
mouse	WB	Mol Med Rep; miR‑153 promotes neural differentiation in the mouse hippocampal HT‑22 cell line and increases the expression of neuron‑specific enolase.; Authors - Chunli Xu