ENO1 Recombinant antibody, PBS Only

ENO1 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C20

Cat no : 81478-1-PBS

Synonyms

ENO1L1, EC:4.2.1.11, C-myc promoter-binding protein, Alpha-enolase, 2-phospho-D-glycerate hydro-lyase

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Validation Data Gallery

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  WB analysis using 81478-1-RR (same clone as 81478-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81478-1-RR (ENO1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81478-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 81478-1-RR (same clone as 81478-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81478-1-RR (ENO1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81478-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 81478-1-RR (same clone as 81478-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 81478-1-RR (ENO1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81478-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 81478-1-RR (same clone as 81478-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81478-1-RR (ENO1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81478-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 81478-1-RR (same clone as 81478-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81478-1-RR (ENO1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81478-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 81478-1-RR (same clone as 81478-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81478-1-RR (ENO1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81478-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 81478-1-RR (same clone as 81478-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81478-1-RR (ENO1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81478-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 81478-1-RR (same clone as 81478-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using ENO1 antibody (81478-1-RR, Clone: 2C20 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81478-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 81478-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81478-1-RR against Human ENO1 were performed. The affinity constant is below 1 pM.

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Product Information

81478-1-PBS targets ENO1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Pig
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ENO1 fusion protein Ag1692
• Full Name: enolase 1, (alpha)
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC015641
• Gene Symbol: ENO1
• Gene ID (NCBI): 2023
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ENO1, also named as NNE, ENO1L1, MBPB1, MPB1 and MBP1, belongs to the enolase family. ENO1 is a metabolic enzyme involved in the synthesis of pyruvate. It also acts as a plasminogen receptor and mediates the activation of plasmin and extracellular matrix degradation. In tumor cells, ENO1 is up-regulated and supports the Warburg effect; it is expressed at the cell surface, where it promotes cancer invasion, and is subjected to a specific array of post-translational modifications, namely acetylation, methylation and phosphorylation. ENO1 overexpression and post-translational modifications could be of diagnostic and prognostic value in many cancer types. (PMID: 27814656). This antibody is specific to ENO1 and has no cross-reaction with ENO2 and ENO3.