ENO1 Polyclonal antibody, PBS Only

ENO1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 55237-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HeLa using 55237-1-AP (same clone as 55237-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 55237-1-AP (ENO1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55237-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 55237-1-AP (same clone as 55237-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 55237-1-AP (ENO1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55237-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 55237-1-AP (same clone as 55237-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55237-1-AP (ENO1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55237-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 55237-1-AP (same clone as 55237-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55237-1-AP (ENO1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55237-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 55237-1-AP (same clone as 55237-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 55237-1-AP (ENO1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55237-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 55237-1-AP (same clone as 55237-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55237-1-AP (ENO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55237-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 55237-1-AP (same clone as 55237-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55237-1-AP (ENO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55237-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse brain using 55237-1-AP (same clone as 55237-1-PBS)

  IP result of anti-ENO1 (IP:55237-1-AP, 4ug; Detection:55237-1-AP 1:500) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 55237-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 55237-1-AP (same clone as 55237-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 55237-1-AP (ENO1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 55237-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 55237-1-AP (same clone as 55237-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 55237-1-AP (ENO1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 55237-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 55237-1-AP (same clone as 55237-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 55237-1-AP (ENO1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 55237-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 55237-1-AP (same clone as 55237-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55237-1-AP (ENO1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 55237-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 55237-1-AP (same clone as 55237-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55237-1-AP (ENO1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 55237-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 55237-1-AP (same clone as 55237-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas using 55237-1-AP (ENO1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 55237-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 55237-1-AP (same clone as 55237-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas using 55237-1-AP (ENO1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 55237-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 55237-1-AP (same clone as 55237-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas using 55237-1-AP (ENO1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 55237-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 55237-1-AP (same clone as 55237-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas using 55237-1-AP (ENO1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 55237-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 55237-1-AP (same clone as 55237-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 55237-1-AP (ENO1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 55237-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 55237-1-AP (same clone as 55237-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 55237-1-AP (ENO1 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 55237-1-PBS in a different storage buffer formulation.

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Product Information

55237-1-PBS targets ENO1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: enolase 1, (alpha)
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: NM_001428
• Gene Symbol: ENO1
• Gene ID (NCBI): 2023
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ENO1, also named as NNE, ENO1L1, MBPB1, MPB1 and MBP1, belongs to the enolase family. ENO1 is a metabolic enzyme involved in the synthesis of pyruvate. It also acts as a plasminogen receptor and mediates the activation of plasmin and extracellular matrix degradation. In tumor cells, ENO1 is up-regulated and supports the Warburg effect; it is expressed at the cell surface, where it promotes cancer invasion, and is subjected to a specific array of post-translational modifications, namely acetylation, methylation and phosphorylation. ENO1 overexpression and post-translational modifications could be of diagnostic and prognostic value in many cancer types. (PMID: 27814656). This antibody is specific to ENO1 and has no cross reaction with ENO2 and ENO3.