Endoglin/CD105 Polyclonal antibody

Endoglin/CD105 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 28117-1-AP

Synonyms

CD105, END, Endoglin, ENG, HHT1, ORW, ORW1

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Validation Data Gallery

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  WB analysis using 28117-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28117-1-AP (Endoglin/CD105 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 28117-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28117-1-AP (Endoglin/CD105 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 28117-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28117-1-AP (Endoglin/CD105 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 28117-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 28117-1-AP (Endoglin/CD105 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 28117-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 28117-1-AP (Endoglin/CD105 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 28117-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 28117-1-AP (Endoglin/CD105 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 28117-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 28117-1-AP (Endoglin/CD105 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 28117-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28117-1-AP (Endoglin/CD105 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 28117-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28117-1-AP (Endoglin/CD105 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HUVEC cells, human placenta tissue
• Positive IHC detected in: human liver cancer tissue, human renal cell carcinoma tissue, human breast cancer tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

28117-1-AP targets Endoglin/CD105 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Endoglin/CD105 fusion protein Ag27822
• Full Name: endoglin
• Calculated Molecular Weight: 71 kDa
• Observed Molecular Weight: 95 kDa
• GenBank Accession Number: BC014271
• Gene Symbol: CD105
• Gene ID (NCBI): 2022
• RRID: AB_2881066
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 90-95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodelling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)

Publications

Species	Application	Title
human	IHC	Mol Cancer Res; A TGFβ-dependent stromal subset underlies immune checkpoint inhibitor efficacy in DNA mismatch repair-deficient/microsatellite instability-high colorectal cancer.; Authors - Eisei Endo
human	IHC	Sci Rep; Public RNA-seq data-based identification and functional analyses reveal that MXRA5 retains proliferative and migratory abilities of dental pulp stem cells; Authors - Kazuma Yoshida
human	IF	Biochem Pharmacol; H3K18 lactylation-mediated VCAM1 expression promotes gastric cancer progression and metastasis via AKT-mTOR-CXCL1 axis; Authors - Yupeng Zhao