ENDOG Polyclonal antibody

• Featured Product
• KD/KO Validated

ENDOG Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

11

Reviews (1)

Cat no : 22148-1-AP

Synonyms

Endonuclease G, Endo G, EC:3.1.30.-

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Validation Data Gallery

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  WB analysis using 22148-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22148-1-AP (ENDOG antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 22148-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 22148-1-AP (ENDOG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 22148-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 22148-1-AP (ENDOG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 22148-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 22148-1-AP (ENDOG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 22148-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22148-1-AP (ENDOG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 22148-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 22148-1-AP (ENDOG Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 22148-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 22148-1-AP (ENDOG Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

• 

  IP experiment of mouse heart using 22148-1-AP

  IP result of anti-ENDOG (IP:22148-1-AP, 4ug; Detection:22148-1-AP 1:1000) with mouse heart tissue lysate 4000ug.

• 

  IHC staining of human ovary cancer using 22148-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 22148-1-AP (ENDOG antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse heart tissue, mouse kidney tissue, mouse liver tissue, mouse skeletal muscle tissue, rat kidney tissue, rat liver tissue
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

22148-1-AP targets ENDOG in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ENDOG fusion protein Ag17739
• Full Name: endonuclease G
• Calculated Molecular Weight: 297 aa, 33 kDa
• Observed Molecular Weight: 27-30 kDa
• GenBank Accession Number: BC016351
• Gene Symbol: ENDOG
• Gene ID (NCBI): 2021
• RRID: AB_11232230
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Endonuclease G, also named as EndoG, is a mitochondrial protein. It's a nuclease which was first characterized in bovine heart mitochondrial extracts. It's involved in many cellular process, including apoptosis, paternal mitochondrial elimination and autophage (PMID:33473107). It is a nuclear encoded, sugar-non-specific (PMID:15066427) and well-conserved nuclease (PMID:17244531). It can be released from the mitochondria and translocated to the nucleus where it induces fragmentation of DNA, leading to apoptosis (PMID:11452314). EndoG is a 297-amino-acid long protein with a molecular weight of 30-35 kDa. There is a homodimer form with MW about 60-70 kDa.

Publications

Species	Application	Title
human	WB	Chem Biol Interact; O-Alkylated derivatives of quercetin induce apoptosis of MCF-7 cells via a caspase-independent mitochondrial pathway.; Authors - Han Liao
rat	WB	Inflammation; Chlorogenic Acid Alleviates Hepatic Ischemia-Reperfusion Injury by Inhibiting Oxidative Stress, Inflammation, and Mitochondria-Mediated Apoptosis In Vivo and In Vitro; Authors - Kai Li
human	WB	Front Pharmacol; Quercitrin Attenuates Acetaminophen-Induced Acute Liver Injury by Maintaining Mitochondrial Complex I Activity.; Authors - Weichen Xiong
human	WB	Front Pharmacol; Emodin Induced SREBP1-Dependent and SREBP1-Independent Apoptosis in Hepatocellular Carcinoma Cells.; Authors - Nian Yang
rat	WB	Metallomics; Induction of mitochondrial apoptosis pathway mediated through caspase-8 and c-Jun N-terminal kinase by cadmium-activated Fas in rat cortical neurons.; Authors - Shuangquan Wen
human	WB	Int J Mol Sci; Proteomics Analysis of Tangeretin-Induced Apoptosis through Mitochondrial Dysfunction in Bladder Cancer Cells.; Authors - Jen-Jie Lin

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Lana (Verified Customer) (06-11-2021)

SDS-PAGE: 40 ug/ul RIPA protein lysate, 4-12% Bis-Tris gradient gel. Transfer: Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4C. Blocking: SEA Block Blocking Buffer 1h, room T. Primary Ab: O/N incubation at 4C, 1:1000. Secondary Ab: IRDye 680LT Goat anti-Rabbit, 1:15000. Lines of WB image: 1 – protein ladder, 2 – mitochondria fraction lysate.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mitochondrial fraction from mice brain tissue

ENDOG Antibody Western Blot validation (1:1000 dilution) in Mitochondrial fraction from mice brain tissue (Cat no:22148-1-AP)