EMR2 Recombinant antibody

• 

  WB analysis using 84608-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84608-5-RR (EMR2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 84608-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84608-5-RR against Human EMR2 were performed. The affinity constant is 1.45 nM.

EMR2 Recombinant Antibody for WB, ELISA

Cat No. 84608-5-RR

Clone No.242042E3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

EGF-like module receptor 2, EGF like module receptor 2, CD312, Adhesion G protein-coupled receptor E2, ADGRE2

---

Tested Applications

• Positive WB detected in: HeLa cells, THP-1 cells, L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84608-5-RR targets EMR2 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: egf-like module containing, mucin-like, hormone receptor-like 2
• Calculated Molecular Weight: 90 kDa
• Observed Molecular Weight: 75~90 kDa
• GenBank Accession Number: NM_013447.3
• Gene Symbol: EMR2
• Gene ID (NCBI): 30817
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9UHX3-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EMR2 (Epidermal Growth Factor-like module-containing Mucin-like Hormone Receptor 2), also known as ADGRE2, is a member of the adhesion-GPCR family that contains extracellular EGF-like domains. EMR2 contains a total of five tandem EGF-like domains and expresses similar protein isoforms consisting of various numbers of EGF-like domains as a result of alternative RNA splicing (PMID: 10903844). EMR2 is restricted to myeloid cells including monocytes, macrophages, dendritic cells and granulocytes28 and its expression is highly regulated during monocyte/macrophage differentiation (PMID: 31969668).The ligation of EMR2 could increase neutrophil adhesion, migration and anti-microbial mediator production and could enhance the systemic inflammation (PMID: 27905560).