Emerin Recombinant antibody

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• KD/KO Validated

Emerin Uni-rAb^TM Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230089B12

Cat no : 82888-3-RR

Synonyms

EMD, STA, LEMD5, EDMD, 230089B12

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Validation Data Gallery

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  WB analysis using 82888-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82888-3-RR (Emerin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 82888-3-RR

  WB result of Emerin antibody (82888-3-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Emerin transfected HeLa cells.

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  IHC staining of human stomach cancer using 82888-3-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82888-3-RR (Emerin antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

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  FC experiment of HeLa using 82888-3-RR

  1x10^6 HeLa cells were intracellularly stained with 0.2 ug EMD Recombinant antibody (82888-3-RR, Clone:230089B12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 82888-3-RR

  Biolayer interferometry (BLl) kinetic assays of 82888-3-RR against Human EMD were performed. The affinity constant is 11.1 nM.

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, Jurkat cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 82888-3-RR is Emerin Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Emerin fusion protein Ag0236
• Full Name: emerin
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC000738
• Gene Symbol: EMD
• Gene ID (NCBI): 2010
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.