ELOVL4 Recombinant antibody

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  FC experiment of A431 using 84050-1-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug Elovl4 Recombinant Antibody (84050-1-RR, Clone:240608A11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

ELOVL4 {ptg:Brand} Recombinant Antibody for FC (Intra), ELISA

Cat No. 84050-1-RR

Clone No.240608A11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra), ELISA

Synonyms

ELOVL fatty acid elongase 4, ELOVL FA elongase 4, EC:2.3.1.199, ADMD, 3-keto acyl-CoA synthase ELOVL4

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Tested Applications

• Positive FC (Intra) detected in: A431 cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84050-1-RR targets ELOVL4 in FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: elongation of very long chain fatty acids (FEN1/Elo2, SUR4/Elo3, yeast)-like 4
• Calculated Molecular Weight: 37 kDa
• GenBank Accession Number: NM_022726
• Gene Symbol: ELOVL4
• Gene ID (NCBI): 6785
• RRID: AB_3671619
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9GZR5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ELOVL4, also named as ADMD, STGD2 and STGD3, belongs to the ELO family. It is a component of the fatty acid elongation system. Elovl4 is involved in the biosynthesis of very long chain fatty acids. It seems to represent a photoreceptor-specific component of the fatty acid elongation system residing on the endoplasmic reticulum. ELOVL4 may be implicated in docosahexaenoic acid (DHA) biosynthesis, which requires dietary consumption of the essential alpha-linolenic acid and a subsequent series of three elongation steps. Defects in ELOVL4 are the cause of Stargardt disease type 3 (STGD3). The antibody is specific to ELOVL4.