ELF5 Polyclonal antibody

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  WB analysis of T-47D using 13107-1-AP

  T-47D cells were subjected to SDS PAGE followed by western blot with 13107-1-AP (ELF5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

ELF5 Polyclonal Antibody for WB, ELISA

Cat No. 13107-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

E74 like factor 5, ELF5, ESE 2, ESE2

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Tested Applications

• Positive WB detected in: T-47D cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13107-1-AP targets ELF5 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ELF5 fusion protein Ag3762
• Full Name: E74-like factor 5 (ets domain transcription factor)
• Calculated Molecular Weight: 255 aa, 30 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: BC029743
• Gene Symbol: ELF5
• Gene ID (NCBI): 2001
• RRID: AB_3669152
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UKW6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ELF5 is an epithelium-specific ETS family transcription factor. In addition to its role in regulating the later stages of terminal differentiation of keratinocytes, it appears to regulate a number of epithelium-specific genes found in tissues containing glandular epithelium such as salivary gland and prostate. It has very low affinity to DNA due to its negative regulatory domain at the amino terminus.