ELF1 Polyclonal antibody

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  WB analysis using 22565-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22565-1-AP (ELF1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 22565-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 22565-1-AP (ELF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 22565-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 22565-1-AP (ELF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 22565-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 22565-1-AP (ELF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 22565-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22565-1-AP (ELF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 22565-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 22565-1-AP (ELF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 22565-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22565-1-AP (ELF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 22565-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 22565-1-AP (ELF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 22565-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22565-1-AP (ELF1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 22565-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22565-1-AP (ELF1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 22565-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22565-1-AP (ELF1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 22565-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 22565-1-AP (ELF1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 22565-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 22565-1-AP (ELF1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 22565-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22565-1-AP (ELF1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 22565-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22565-1-AP (ELF1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of PC-3 using 22565-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed PC-3 cells using 22565-1-AP (ELF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

ELF1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 22565-1-AP

Publications(9)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, chIP, ELISA, EMSA

Synonyms

ETS-related transcription factor Elf-1, E74-like factor 1, E74 like factor 1

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Tested Applications

• Positive WB detected in: PC-3 cells, HeLa cells, A431 cells, Jurkat cells, HL-60 cells, mouse thymus tissue
• Positive IHC detected in: human colon cancer tissue, human pancreas cancer tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:100-1:1200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• ChIP: See 2 publications below

Product Information

22565-1-AP targets ELF1 in WB, IHC, IF/ICC, chIP, ELISA, EMSA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ELF1 fusion protein Ag14689
• Full Name: E74-like factor 1 (ets domain transcription factor)
• Calculated Molecular Weight: 619 aa, 67 kDa
• Observed Molecular Weight: 97 kDa
• GenBank Accession Number: BC030507
• Gene Symbol: ELF1
• Gene ID (NCBI): 1997
• RRID: AB_2879123
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P32519
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ELF1, also named as ETS-related transcription factor Elf-1, is originally cloned from a human T-cell cDNA library by hybridization with a probe encoding the DNA binding domain (ETS domain) of the human Ets-1 cDNA. Based on its preferential expression in embryonic lymphoid organs (thymus and spleen), a wide variety of epithelial cells and fetal liver as well as in adult haematopoietic tissues, including thymus, spleen and bone marrow, Elf-1 emerged as a potential key regulator of haematopoietic gene expression. Consistent with this notion, Elf-1 has been shown to be a direct upstream regulator of genes important for haematopoiesis such as Scl, Fli-1, Lyl-1, Runx1 and Lmo2 . Elf-1 has also been shown to be important for blood vessel development, a process that is closely linked to early haematopoiesis during embryonic development. Elf-1 has been reported to take part in the transcriptional control of major regulators of blood vessel development such as Tie1, Tie2, angiopoietin-2, the vascular endothelial growth factor receptor 1 (VEGFR1), the endothelial nitric-oxide synthase (eNOS) and endoglin . Functional activity of Ets proteins is modulated at multiple levels. It is known that ELF-1 appears in the cytoplasm as a 80 KDa protein that is O -glycosylated and phosphorylated in order to be translocated into the nucleus where it can be detected as a 98 KDa protein. After dephosphorylation, the protein is degraded through the proteasome pathway. The inactive form of Elf-1 is an 80-kDa protein that lacks DNA-binding activity and is confined to the cytoplasm of the cell. Phosphorylation and O-linked glycosylation increase the molecular weight of Elf-1 to 98 kDa, the active form; 98 kDa Elf-1 binds to the promoter of the gene that codes for CD3ζ inducing its transcription.

Publications

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
human	EMSA	J Autoimmun; rs9459874 and rs1012656 in CCR6/FGFR1OP confer susceptibility to primary biliary cholangitis.; Authors - Yuki Hitomi
mouse	WB,IF,ChIP	Biochim Biophys Acta Mol Basis Dis; ARL11 knockdown alleviates spinal cord injury by inhibiting neuroinflammation and M1 activation of microglia in mice; Authors - Haocong Zhang
human	WB	Front Bioeng Biotechnol; Let-7i-5p Regulation of Cell Morphology and Migration Through Distinct Signaling Pathways in Normal and Pathogenic Urethral Fibroblasts.; Authors - Kaile Zhang
human	chIP	J Exp Clin Cancer Res; TNFAIP8 promotes AML chemoresistance by activating ERK signaling pathway through interaction with Rac1.; Authors - Yihua Pang
human	IHC	BMC Cancer; Novel biomarkers to predict treatment response and prognosis in locally advanced rectal cancer undergoing neoadjuvant chemoradiotherapy; Authors - Bingjie Guan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Iru (Verified Customer) (06-18-2020)

There are other non-specific multiple bands as well.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Fallopian tube epithelial cells