ELAVL4 Monoclonal antibody, PBS Only

ELAVL4 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat, mouse, rabbit, pig

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B1F5

Cat no : 67835-1-PBS

Synonyms

HUD, Hu-antigen D, HuA/B/C/D, Hu C/D, ELAV-like protein 4

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Validation Data Gallery

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  WB analysis of U2OS using 67835-1-Ig (same clone as 67835-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 67835-1-Ig (ELAVL4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67835-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 67835-1-Ig (same clone as 67835-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 67835-1-Ig (ELAVL4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67835-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67835-1-Ig (same clone as 67835-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67835-1-Ig (ELAVL4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67835-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67835-1-Ig (same clone as 67835-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67835-1-Ig (ELAVL4 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67835-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67835-1-Ig (same clone as 67835-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67835-1-Ig (ELAVL4 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67835-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 67835-1-Ig (same clone as 67835-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ELAVL4 antibody (67835-1-Ig, Clone: 1B1F5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67835-1-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 67835-1-Ig (same clone as 67835-1-PBS)

  1X10^6 U2OS cells were intracellularly stained with 0.4 ug Anti-Human ELAVL4 (67835-1-Ig, Clone:1B1F5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67835-1-PBS in a different storage buffer formulation.

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Product Information

67835-1-PBS targets ELAVL4 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with Human, rat, mouse, rabbit, pig samples.

• Tested Reactivity: Human, rat, mouse, rabbit, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: ELAVL4 fusion protein Ag3678
• Full Name: ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D)
• Calculated Molecular Weight: 380 aa, 42 kDa
• Observed Molecular Weight: 32-40 kDa
• GenBank Accession Number: BC036071
• Gene Symbol: ELAVL4
• Gene ID (NCBI): 1996
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ELAVL4, also named as HuD, is a 380 amino acid protein. The full name is neuronal embryonic lethal altered visual system-like(ELAVL) RNA-binding protein. ELAVL4 is expressed in brain and localized in the nucleus. PC12 cells expressing T7±HuD were treated with thio-specific (BMH) or amine-specific (DSS) chemical crosslinking reagents, and the resultant crosslinked complexes in the cell extracts were analyzed by immunoblotting with anti-T7 antibody. In addition to the monomeric HuD of 41 kDa, three specific crosslinked complexes could be detected using either of the crosslinkers. Two of the complexes had molecular masses of ~80 and 120 kDa, and were likely to be the dimer and trimer of HuD, respectively. (PMID: 12384599 )