HuA/B/C/D Polyclonal antibody

HuA/B/C/D Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 13032-1-AP

Synonyms

HUD, ELAVL4, ELAV like protein 4, Hu-antigen D, Hu C/D

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Validation Data Gallery

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  WB analysis of human cerebellum using 13032-1-AP

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 13032-1-AP (HuA/B/C/D antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-13 using 13032-1-AP

  PC-13 cells were subjected to SDS PAGE followed by western blot with 13032-1-AP (HuA/B/C/D antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-13 using 13032-1-AP

  PC-13 cells were subjected to SDS PAGE followed by western blot with 13032-1-AP (HuA/B/C/D antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse cerebellum using 13032-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 13032-1-AP (HuA/B/C/D antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13032-1-AP

  IP result of anti-HuA/B/C/D (IP:13032-1-AP, 4ug; Detection:13032-1-AP 1:300) with mouse brain tissue lysate 2560ug.

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  IHC staining of human brain using 13032-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13032-1-AP (HuA/B/C/D antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human brain using 13032-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13032-1-AP (HuA/B/C/D antibody at dilution of 1:200 (under 10x lens).

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  IF Staining of mouse brain using 13032-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using HuA/B/C/D antibody (13032-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human cerebellum tissue, mouse cerebellum tissue, PC-13 cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

13032-1-AP targets HuA/B/C/D in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HuA/B/C/D fusion protein Ag3678
• Full Name: ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D)
• Calculated Molecular Weight: 380 aa, 42 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC036071
• Gene Symbol: ELAVL4
• Gene ID (NCBI): 1996
• RRID: AB_2096385
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Embryonic lethal, abnormal vision, Drosophila-like 4 (ELAVL4) is a human homologue of the Drosophilia gene ELAV, which is implicated in neuronal differentiation and maintenance. The ELAVL proteins are mRNA binding proteins and all members of the family contain three RNA-recognition motifs [PMID:12402251]. ELAVL-proteins also interact with inflammation-associated factors. The protein may be implicated in neurodegeneration through these complex mechanisms [PMID:15827745].

Publications

Species	Application	Title
rat	IHC	J Neurosci Methods; Efficient infection of organotypic hippocampal slice cultures with adenovirus carrying the transgene REST/NRSF.; Authors - Ruth Butler-Ryan
human	IHC,WB	J Cosmet Dermatol; Study on the correlation of high expression level of HuD with seborrheic keratosis and skin aging; Authors - Juping Chen
mouse	IF	Med Mol Morphol; Altered microbiota caused by disordered gut motility leads to an overactivation of intestinal immune system in APC1638T mice; Authors - Nami O Yamada
mouse	IF	Biomed Pharmacother; Shouhui Tongbian Capsule ameliorates constipation via gut microbiota-5-HT-intestinal motility axis; Authors - Jinzhao Bai