EIF5B Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

EIF5B Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13527-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of mouse brain using 13527-1-AP (same clone as 13527-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13527-1-AP (EIF5B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13527-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 13527-1-AP (same clone as 13527-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 13527-1-AP (EIF5B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13527-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 13527-1-AP (same clone as 13527-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13527-1-AP (EIF5B antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13527-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 13527-1-AP (same clone as 13527-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13527-1-AP (EIF5B antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13527-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 13527-1-AP (same clone as 13527-1-PBS)

  Immunofluorescent analysis of MCF-7 cells, using EIF5B antibody 13527-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 13527-1-PBS in a different storage buffer formulation.

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Product Information

13527-1-PBS targets EIF5B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EIF5B fusion protein Ag4404
• Full Name: eukaryotic translation initiation factor 5B
• Calculated Molecular Weight: 1220 aa, 139 kDa
• Observed Molecular Weight: 175 kDa
• GenBank Accession Number: BC032639
• Gene Symbol: EIF5B
• Gene ID (NCBI): 9669
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Translation initiation requires the delivery of the initiator methionine tRNA to the 40S ribosomal subunit. The initiator methionine tRNA is delivered by the heterotrimeric complex EIF2 in a ternary complex with GTP that interacts with the 40S subunit. The resulting complex then binds to an mRNA and scans for the AUG start codon. Eukaryotic translation initiation factor 5B (EIF5B) plays a role in recognition of the AUG codon in conjunction with translation factor eIF2, which functions to general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes, and promotes joining of the 60S ribosomal subunit. A single crossreactive polypeptide of 175 kDa was detected, whereas the predicted size of the protein was 139 kDa. This size discrepancy may be the result of posttranslational modifications of EIF5B or, perhaps more likely, of unusual behavior in SDS-PAGE caused by the highly charged N-terminal region of EIF5B (PMID: 10200264 ).