EIF5A Polyclonal antibody

• Featured Product
• KD/KO Validated

EIF5A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 11309-1-AP

Synonyms

eIF-5A, eIF-4D, eIF 5A1, eIF 5A 1, EIF 5A

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Validation Data Gallery

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  WB analysis using 11309-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11309-1-AP (EIF5A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11309-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11309-1-AP (EIF5A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11309-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11309-1-AP (EIF5A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11309-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11309-1-AP (EIF5A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 11309-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11309-1-AP (EIF5A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 11309-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11309-1-AP (EIF5A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 11309-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EIF5A antibody (11309-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, NIH/3T3 cells, PC-3 cells, SH-SY5Y cells, mouse brain tissue
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

11309-1-AP targets EIF5A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EIF5A fusion protein Ag1849
• Full Name: eukaryotic translation initiation factor 5A
• Calculated Molecular Weight: 18 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC001832
• Gene Symbol: EIF5A
• Gene ID (NCBI): 1984
• RRID: AB_2262001
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Translation of messenger RNA (mRNA) to protein in eukaryotesis a crucial process in protein biosynthesis, in which initiation of translation involves interaction of different eukaryotic translation initiation factors (eIFs), ribosome subunits and mRNAs. Eukaryotic translation initiation factor 5A (EIF5A) is one of the eIFs involved in translation initiation. EIF5A has a essential role in cell viability and the only protein known to contain the amino acid residue hypusine, formed by post-translational modification of a specific lysine residue. Firstly identified as a translation initiation factor, it also has a function in the elongation step of translation.

Publications

Species	Application	Title
human	WB,IHC	J Cancer; EIF5A expression and its role as a potential diagnostic biomarker in hepatocellular carcinoma.; Authors - Linjing Li
mouse	IF	J Ovarian Res; Proteomic analysis implicates that postovulatory aging leads to aberrant gene expression, biosynthesis, RNA metabolism and cell cycle in mouse oocytes; Authors - Chuanxin Zhang
human	WB	Oncol Lett; SHCBP1 contributes to the proliferation and self‑renewal of cervical cancer cells and activation of the NF‑κB signaling pathway through EIF5A; Authors - Boya Deng
human	WB	Nat Chem Biol; ENO1 promotes liver carcinogenesis through YAP1-dependent arachidonic acid metabolism; Authors - Linchong Sun ; KD Validated