EIF4E3 Polyclonal antibody

• Featured Product
• KD/KO Validated

EIF4E3 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 17282-1-AP

Synonyms

eIF-4E3, eIF-4E type 3, eIF4E type 3, eIF4E 3, eIF 4E3

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Validation Data Gallery

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  WB analysis of HeLa using 17282-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17282-1-AP (EIF4E3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17282-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17282-1-AP (EIF4E3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 17282-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 17282-1-AP (EIF4E3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 17282-1-AP

  Immunofluorescent analysis of Hela cells, using EIF4E3 antibody 17282-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells, mouse heart tissue, HepG2 cells
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

17282-1-AP targets EIF4E3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EIF4E3 fusion protein Ag11056
• Full Name: eukaryotic translation initiation factor 4E family member 3
• Calculated Molecular Weight: 24 kDa
• Observed Molecular Weight: 24-30 kDa
• GenBank Accession Number: BC031289
• Gene Symbol: EIF4E3
• Gene ID (NCBI): 317649
• RRID: AB_2262162
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EIF4E3, also known as Eukaryotic translation initiation factor 4E type 3, is a 224 amino acid protein, which recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis. EIF4E3 acts as a tissue-specific tumor suppressor. For instance, eIF4E3 inhibits expression of both mRNA export and translation targets of eIF4E1, consistent with its nuclear and cytoplasmic localization. Importantly, eIF4E3 overexpression represses oncogenic transformation. (PMID: 23587918).

Publications

Species	Application	Title
mouse	WB,IF	Nat Commun; PMID: 30382096; Authors - Nourhan Abdelfattah ; KD Validated
human	WB, IP	Nat Commun; MNKs act as a regulatory switch for eIF4E1 and eIF4E3 driven mRNA translation in DLBCL.; Authors - Ari L Landon
human	IHC	Proc Natl Acad Sci U S A; Local translation in nuclear condensate amyloid bodies.; Authors - Phaedra R Theodoridis
human	WB	Mol Ther; RNA m6A methylation regulates the dissemination of cancer cells via modulating expression and membrane localization of β-catenin.; Authors - Jiexin Li
human	WB	iScience; Transcriptome, proteome, and protein synthesis within the intracellular cytomatrix; Authors - Tattym E Shaiken
human	WB	Oncotarget; Noncanonical SQSTM1/p62-Nrf2 pathway activation mediates proteasome inhibitor resistance in multiple myeloma cells via redox, metabolic and translational reprogramming.; Authors - Irene Riz ; KD Validated