EIF4B Polyclonal antibody

• Featured Product
• KD/KO Validated

EIF4B Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 17917-1-AP

Synonyms

PRO1843, eIF-4B, EIF 4B

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Validation Data Gallery

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  WB analysis using 17917-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17917-1-AP (EIF4B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17917-1-AP

  WB result of EIF4B antibody (17917-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EIF4B transfected HeLa cells.

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  WB analysis of HeLa using 17917-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17917-1-AP (EIF4B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 17917-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 17917-1-AP (EIF4B antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 17917-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using EIF4B antibody (17917-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 17917-1-AP

  Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using EIF4B antibody (17917-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  FC experiment of HeLa using 17917-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human EIF4B (17917-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HeLa cells, K-562 cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells, sodium arsenite treated HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

17917-1-AP targets EIF4B in WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EIF4B fusion protein Ag12325
• Full Name: eukaryotic translation initiation factor 4B
• Calculated Molecular Weight: 611 aa, 69 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC073154
• Gene Symbol: EIF4B
• Gene ID (NCBI): 1975
• RRID: AB_2097541
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EIF4B is one of the mammalian eukaryotic initiation factors (eIF) that are required for the ATP-dependent binding of mRNA to the 40 S ribosomal subunit, and the other eIF proteins are EIF4A, EIF4F. eIF4B is involved in translation of numerous proliferative or anti-apoptotic mRNAs with highly structured 5'UTR and subsequently affect cell growth and survival. It was reported that false expression and phosphorylation levels of eIF4B are involved in several tumors including breast cancer, cell lymphoblastic leukemia and diffuse large B-cell lymphoma (PMID: 26848623).

Publications

Species	Application	Title
	WB	Cell; Circadian clocks are modulated by compartmentalized oscillating translation; Authors - Yanrong Zhuang
human	WB	Brain; MMP13 inhibition rescues cognitive decline in Alzheimer transgenic mice via BACE1 regulation.; Authors - Bing-Lin Zhu
rat	WB	Nucleic Acids Res; PMID: 30295850; Authors - Zonghong Li
	WB	Mol Med Rep; MicroRNA‑216a inhibits the growth and metastasis of oral squamous cell carcinoma by targeting eukaryotic translation initiation factor 4B.; Authors - Lei Li
human	WB	Biotechnol J; Highly efficient identification of nucleocytoplasmic O-glycosylation by the TurboID-based proximity labeling method in living cells; Authors - Yunfeng Long
human	WB,IF,CoIP	Biochem Pharmacol; Phytic acid promotes high glucose-mediated bone marrow mesenchymal stem cells osteogenesis via modulating circEIF4B that sponges miR-186-5p and complexes with IGF2BP3; Authors - Jin Wu