EIF4A3 Monoclonal antibody, PBS Only

EIF4A3 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G6H9

Cat no : 67740-1-PBS

Synonyms

DDX48, DEAD box protein 48, eIF 4A III, eIF4A III, EIF4A3, eIF4AIII, hNMP 265, hNMP265, KIAA0111, Nuclear matrix protein 265, NUK 34

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Validation Data Gallery

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  WB analysis using 67740-1-Ig (same clone as 67740-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67740-1-Ig (EIF4A3 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67740-1-PBS in a different storage buffer formulation.

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  WB analysis using 67740-1-Ig (same clone as 67740-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67740-1-Ig (EIF4A3 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67740-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67740-1-Ig (same clone as 67740-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67740-1-Ig (EIF4A3 antibody) at dilution of 1:2500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67740-1-PBS in a different storage buffer formulation.

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  IHC staining of human renal cell carcinoma using 67740-1-Ig (same clone as 67740-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 67740-1-Ig (EIF4A3 antibody) at dilution of 1:2500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67740-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67740-1-Ig (same clone as 67740-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67740-1-Ig (EIF4A3 antibody) at dilution of 1:2500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67740-1-PBS in a different storage buffer formulation.

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  IHC staining of human renal cell carcinoma using 67740-1-Ig (same clone as 67740-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 67740-1-Ig (EIF4A3 antibody) at dilution of 1:2500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67740-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67740-1-Ig (same clone as 67740-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using EIF4A3 antibody (67740-1-Ig, Clone: 1G6H9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67740-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67740-1-PBS targets EIF4A3 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: EIF4A3 fusion protein Ag11130
• Full Name: eukaryotic translation initiation factor 4A, isoform 3
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC003662
• Gene Symbol: EIF4A3
• Gene ID (NCBI): 9775
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

EIF4A3 is a component of the exon junction complex (EJC), which assembles near exon-exon junctions of mRNAs as a result of splicing. EJC proteins involves in postsplicing events, including mRNA export, cytoplasmic localization, and nonsense-mediated decay. Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH/RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. Besides, it involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex and binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions