EIF4A3 Polyclonal antibody, PBS Only

EIF4A3 Polyclonal Antibody for WB, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10463-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HeLa using 10463-1-AP (same clone as 10463-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10463-1-AP (EIF4A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10463-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 10463-1-AP (same clone as 10463-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10463-1-AP (EIF4A3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10463-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 10463-1-AP (same clone as 10463-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10463-1-AP (EIF4A3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10463-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 10463-1-AP (same clone as 10463-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10463-1-AP (EIF4A3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10463-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Raji using 10463-1-AP (same clone as 10463-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 10463-1-AP (EIF4A3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10463-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 10463-1-AP (same clone as 10463-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10463-1-AP (EIF4A3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10463-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 10463-1-AP (same clone as 10463-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10463-1-AP (EIF4A3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10463-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 10463-1-AP (same clone as 10463-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10463-1-AP (EIF4A3 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10463-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human lung using 10463-1-AP (same clone as 10463-1-PBS)

  human lung tissue were subjected to SDS PAGE followed by western blot with 10463-1-AP (EIF4A3 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10463-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 10463-1-AP (same clone as 10463-1-PBS)

  IP result of anti-EIF4A3 (IP:10463-1-AP, 3ug; Detection:10463-1-AP 1:300) with HEK-293 cells lysate 2800ug. This data was developed using the same antibody clone with 10463-1-PBS in a different storage buffer formulation.

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Product Information

10463-1-PBS targets EIF4A3 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EIF4A3 fusion protein Ag0733
• Full Name: eukaryotic translation initiation factor 4A, isoform 3
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC004386
• Gene Symbol: EIF4A3
• Gene ID (NCBI): 9775
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

EIF4A3 is a component of the exon junction complex (EJC), which assembles near exon-exon junctions of mRNAs as a result of splicing. EJC proteins involves in postsplicing events, including mRNA export, cytoplasmic localization, and nonsense-mediated decay. Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH/RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. Besides, it involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex and binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions