EIF4A2 Polyclonal antibody

EIF4A2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 11280-1-AP

Synonyms

BM 010, DDX2B, eIF 4A II, EIF4A, eIF4A II, EIF4A2, EIF4F

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Validation Data Gallery

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  WB analysis of Jurkat using 11280-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11280-1-AP (EIF4A2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 11280-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 11280-1-AP (EIF4A2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 11280-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 11280-1-AP (EIF4A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11280-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11280-1-AP (EIF4A2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 11280-1-AP

  IP result of anti-EIF4A2 (IP:11280-1-AP, 4ug; Detection:11280-1-AP 1:500) with Jurkat cells lysate 4000ug.

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  IHC staining of human breast cancer using 11280-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11280-1-AP (EIF4A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11280-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11280-1-AP (EIF4A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 11280-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11280-1-AP (EIF4A2 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: Jurkat cells, mouse ovary tissue, Raji cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human breast cancer tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

11280-1-AP targets EIF4A2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EIF4A2 fusion protein Ag1810
• Full Name: eukaryotic translation initiation factor 4A, isoform 2
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC015842
• Gene Symbol: EIF4A2
• Gene ID (NCBI): 1974
• RRID: AB_2097378
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Eukaryotic initiation factor 4A (EIF4A) has an essential role in the binding of mRNA to the 43S preinitiation complex when protein synthesis begins, as EIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. EIF4A2 is a ATP-dependent RNA helicase, which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome.

Publications

Species	Application	Title
human	IHC, WB	Proc Natl Acad Sci U S A; Local translation in nuclear condensate amyloid bodies.; Authors - Phaedra R Theodoridis
rat	WB	Mol Ther Nucleic Acids; Translatome and Transcriptome Profiling of Hypoxic-Induced Rat Cardiomyocytes.; Authors - Zhijie Shen
rat	WB	Mol Neurobiol; Identification of Immune-Related Biomarkers of Schizophrenia in the Central Nervous System Using Bioinformatic Methods and Machine Learning Algorithms; Authors - Jianjun Weng
mouse	WB	Stem Cell Rev Rep; Hyaluronan-CD44 Interaction Regulates Mouse Retinal Progenitor Cells Migration, Proliferation and Neuronal Differentiation; Authors - Jian Ma
human	WB	Mol Carcinog; miR-181a/b Induces the Growth, Invasion and Metastasis of Neuroblastoma Cells Through Targeting ABI1.; Authors - Xiaodan Liu
mouse	WB,IF	Biol Reprod; Nuclear localization of EIF4G3 suggests a role for the XY body in translational regulation during spermatogenesis in mice.; Authors - Jianjun Hu