EIF3M Monoclonal antibody, PBS Only

EIF3M Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A12B2

Cat no : 66025-1-PBS

Synonyms

B5, EIF3M, Fetal lung protein B5, GA17, hfl B5, HFLB5, PCID1, PNAS 125

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Validation Data Gallery

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  WB analysis using 66025-1-Ig (same clone as 66025-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66025-1-Ig (EIF3M antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66025-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66025-1-Ig (same clone as 66025-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66025-1-Ig (EIF3M antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66025-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66025-1-Ig (same clone as 66025-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66025-1-Ig (EIF3M antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66025-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 66025-1-Ig (same clone as 66025-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 66025-1-Ig (EIF3M antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66025-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66025-1-Ig (same clone as 66025-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66025-1-Ig (EIF3M antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66025-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66025-1-Ig (same clone as 66025-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66025-1-Ig (EIF3M antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66025-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 66025-1-Ig (same clone as 66025-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66025-1-Ig (EIF3M antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66025-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 66025-1-Ig (same clone as 66025-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66025-1-Ig (EIF3M antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66025-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 66025-1-Ig (same clone as 66025-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 66025-1-Ig (EIF3M antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66025-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 66025-1-Ig (same clone as 66025-1-PBS)

  Immunofluorescent analysis of MCF-7 cells, using EIF3M antibody 66025-1-lg at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). This data was developed using the same antibody clone with 66025-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66025-1-PBS targets EIF3M in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: EIF3M fusion protein Ag17965
• Full Name: eukaryotic translation initiation factor 3, subunit M
• Calculated Molecular Weight: 374 aa, 43 kDa
• Observed Molecular Weight: 35-43 kDa
• GenBank Accession Number: BC019103
• Gene Symbol: EIF3M
• Gene ID (NCBI): 10480
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

EIF3M gene encodes eukaryotic translation initiation factor (eIF) subunit M, also called GA17 or PCID1. eIF-3 complex is required for several steps in the initiation of protein synthesis, and recent studies indicate that regulation of oncogene expression and neoplastic transformation are controlled by eIF subunits. The most uncharacterized non-core subunit EIF3M was confirmed to be highly expressed in human cancer cell lines and colon cancer patient tissues and mediate regulation of tumorigenesis-related genes in human colon cancer.