HeLa cells were subjected to SDS PAGE followed by western blot with 11352-1-AP (EIF3E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
HeLa cells were subjected to SDS PAGE followed by western blot with 11352-1-AP (EIF3E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 11352-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 11352-1-AP (EIF3E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
HepG2 cells were subjected to SDS PAGE followed by western blot with 11352-1-AP (EIF3E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 11352-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11352-1-AP (EIF3E antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11352-1-AP (EIF3E antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 11352-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 11352-1-AP (EIF3E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Jurkat cells were subjected to SDS PAGE followed by western blot with 11352-1-AP (EIF3E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 11352-1-AP
IP result of anti-EIF3E (IP:11352-1-AP, 4ug; Detection:11352-1-AP 1:500) with HepG2 cells lysate 880 ug.
IP result of anti-EIF3E (IP:11352-1-AP, 4ug; Detection:11352-1-AP 1:500) with HepG2 cells lysate 880 ug.
IHC staining of human breast cancer using 11352-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11352-1-AP (EIF3E antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11352-1-AP (EIF3E antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 11352-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11352-1-AP (EIF3E antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11352-1-AP (EIF3E antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 11352-1-AP
Immunofluorescent analysis of HepG2 cells using 11352-1-AP (EIF3E antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.
Immunofluorescent analysis of HepG2 cells using 11352-1-AP (EIF3E antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.
IF Staining of HepG2 using 11352-1-AP
Immunofluorescent analysis of Ethacrynic acid treated HepG2 cells using 11352-1-AP (EIF3E antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.
Immunofluorescent analysis of Ethacrynic acid treated HepG2 cells using 11352-1-AP (EIF3E antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells
Positive IP detected in
HepG2 cells
Positive IHC detected in
human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HepG2 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:2400
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)/ICC
IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
11352-1-AP targets EIF3E in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
HeLa cells were subjected to SDS PAGE followed by western blot with 11352-1-AP (EIF3E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 11352-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 11352-1-AP (EIF3E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 11352-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11352-1-AP (EIF3E antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 11352-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 11352-1-AP (EIF3E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human breast cancer using 11352-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11352-1-AP (EIF3E antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 11352-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11352-1-AP (EIF3E antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of HepG2 using 11352-1-AP
IP result of anti-EIF3E (IP:11352-1-AP, 4ug; Detection:11352-1-AP 1:500) with HepG2 cells lysate 880 ug.
IF/ICC Figures
IF Staining of HepG2 using 11352-1-AP
Immunofluorescent analysis of HepG2 cells using 11352-1-AP (EIF3E antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.
IF Staining of HepG2 using 11352-1-AP
Immunofluorescent analysis of Ethacrynic acid treated HepG2 cells using 11352-1-AP (EIF3E antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
EIF3E Polyclonal antibody
Catalog Number
11352-1-AP
Citations
-
Dilutions
WB : 1:500-1:2400 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF/ICC : 1:10-1:100
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
