EIF3B Polyclonal antibody

EIF3B Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 10319-1-AP

Synonyms

eIF 3 eta, EIF3 ETA, EIF3 P110, EIF3 P116, EIF3B, EIF3S9, hPrt1, PRT1, Prt1 homolog

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Validation Data Gallery

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  WB analysis of A375 using 10319-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 10319-1-AP (EIF3B Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of A375 using 10319-1-AP

  IP result of anti-EIF3B (IP:10319-1-AP, 4ug; Detection:10319-1-AP 1:300) with A375 cells lysate 3600ug.

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Tested Applications

• Positive WB detected in: A375 cells
• Positive IP detected in: A375 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IF: See 1 publications below

Product Information

10319-1-AP targets EIF3B in WB, IP, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EIF3B fusion protein Ag0386
• Full Name: eukaryotic translation initiation factor 3, subunit B
• Calculated Molecular Weight: 93 kDa
• Observed Molecular Weight: 115 kDa
• GenBank Accession Number: BC001173
• Gene Symbol: EIF3B
• Gene ID (NCBI): 8662
• RRID: AB_2096732
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EIF3B, also named as Eukaryotic translation initiation factor 3 subunit B, is a 814 amino acid protein, which contains 1 RRM (RNA recognition motif) domain and 8 WD repeats and belongs to the eIF-3 subunit B family. EIF3B as a RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. The calculated molecular weight of EIF3B is 93 kDa, but the phosphorylated EIF3B protein is about 115 kDa.

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; SUMOylation of the m6A-RNA methyltransferase METTL3 modulates its function.; Authors - Yuzhang Du
human	WB	Elife; Translation initiation by the hepatitis C virus IRES requires eIF1A and ribosomal complex remodeling.; Authors - Zane A Jaafar
human	WB	Cancers (Basel); GDF15 Is an Eribulin Response Biomarker also Required for Survival of DTP Breast Cancer Cells.; Authors - Chiara Bellio
mouse	WB	bioRxiv; Novel gene-specific translation mechanism of dysregulated, chronic inflammation reveals promising, multifaceted COVID-19 therapeutics.; Authors - Li Wang
human	WB	Nat Commun; METTL3 acetylation impedes cancer metastasis via fine-tuning its nuclear and cytosolic functions; Authors - Yuanpei Li
		Cell Chem Biol; Non-canonical function of histone methyltransferase G9a in the translational regulation of chronic inflammation; Authors - Adil Muneer