EIF3A Polyclonal antibody

EIF3A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27665-1-AP

Synonyms

eIF 3 theta, EIF3, eIF3 p167, eIF3 p170, eIF3 p180, eIF3 p185, eIF3 theta, EIF3A, EIF3S10, KIAA0139, P167, p180, p185, TIF32

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Validation Data Gallery

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  WB analysis of BxPC-3 using 27665-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27665-1-AP (EIF3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27665-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27665-1-AP (EIF3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 27665-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27665-1-AP (EIF3A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 27665-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27665-1-AP (EIF3A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 27665-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 27665-1-AP (EIF3A antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: BxPC-3 cells, SKOV-3 cells, mouse brain tissue
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27665-1-AP targets EIF3A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EIF3A fusion protein Ag24752
• Full Name: eukaryotic translation initiation factor 3, subunit A
• Calculated Molecular Weight: 1382 aa, 166 kDa
• Observed Molecular Weight: 166 kDa
• GenBank Accession Number: BC114429
• Gene Symbol: EIF3A
• Gene ID (NCBI): 8661
• RRID: AB_2880942
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.