EIF2S3 Polyclonal antibody, PBS Only

EIF2S3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11162-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HeLa using 11162-1-AP (same clone as 11162-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 11162-1-AP (EIF2S3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11162-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 11162-1-AP (same clone as 11162-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 11162-1-AP (EIF2S3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11162-1-PBS in a different storage buffer formulation.

• 

  WB analysis of COLO 320 using 11162-1-AP (same clone as 11162-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11162-1-AP (EIF2S3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11162-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse thymus using 11162-1-AP (same clone as 11162-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11162-1-AP (EIF2S3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11162-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse thymus using 11162-1-AP (same clone as 11162-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11162-1-AP (EIF2S3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11162-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 11162-1-AP (same clone as 11162-1-PBS)

  IP result of anti-EIF2S3 (IP:11162-1-AP, 4ug; Detection:11162-1-AP 1:500) with HeLa cells lysate 2800ug. This data was developed using the same antibody clone with 11162-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 11162-1-AP (same clone as 11162-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11162-1-AP (EIF2S3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11162-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 11162-1-AP (same clone as 11162-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11162-1-AP (EIF2S3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11162-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 11162-1-AP (same clone as 11162-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 11162-1-AP (EIF2S3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11162-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 11162-1-AP (same clone as 11162-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11162-1-AP (EIF2S3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11162-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 11162-1-AP (same clone as 11162-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11162-1-AP (EIF2S3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11162-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 11162-1-AP (same clone as 11162-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11162-1-AP (EIF2S3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11162-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 11162-1-AP (same clone as 11162-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11162-1-AP (EIF2S3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11162-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 11162-1-AP (same clone as 11162-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 11162-1-AP (EIF2S3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11162-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 11162-1-AP (same clone as 11162-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 11162-1-AP (EIF2S3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11162-1-PBS in a different storage buffer formulation.

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Product Information

11162-1-PBS targets EIF2S3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EIF2S3 fusion protein Ag1650
• Full Name: eukaryotic translation initiation factor 2, subunit 3 gamma, 52kDa
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC019906
• Gene Symbol: EIF2S3
• Gene ID (NCBI): 1968
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Interacts with GTP and initiator methionyl-tRNA, translation initiation factor eIF2 forms a ternary complex that binds the 40S ribosome and then scans an mRNA to select the AUG start codon for protein synthesis[PMID: 9736774]. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. EIF2S3 is the core subunit of the heterotrimeric eIF2 complex.[PMID:23063529]