EIF2S1 Recombinant antibody, PBS Only (Capture)

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  Sandwich ELISA standard curve of MP00076-4

  Sandwich ELISA standard curve of MP00076-4, Human EIF2S1 Recombinant Matched Antibody Pair - PBS only. 82936-6-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1645. 82936-5-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

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  Sample test of MP00076-4

  The mean EIF2S1 concentration was determined to be 11.12 ng/mL in PC-3 cell extract based on a 1.20 mg/mL extract load.

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  Cytometric bead array standard curve of MP00076-3

  Cytometric bead array standard curve of MP00076-3, EIF2S1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82936-6-PBS. Detection antibody: 82936-7-PBS. Standard: Ag1645. Range: 1.25-20 ng/mL.

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  Affinity and Kinetic Characterization of 82936-6-PBS

  Biolayer interferometry (BLl) kinetic assay of 82936-6-PBS against Human EIF2S1 was performed. The affinity constant is 3.46 nM.

EIF2S1 Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 82936-6-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

EIF2S1/EIF2A, EIF2, EIF 2alpha, EIF 2A, eIF 2 alpha

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Product Information

82936-6-PBS targets EIF2S1 as part of a matched antibody pair:

MP00076-3: 82936-6-PBS capture and 82936-7-PBS detection (validated in Cytometric bead array)

MP00076-4: 82936-6-PBS capture and 82936-5-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: EIF2S1 fusion protein Ag1645
• Full Name: eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
• Calculated Molecular Weight: 36 kDa
• GenBank Accession Number: BC002513
• Gene Symbol: EIF2S1
• Gene ID (NCBI): 1965
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P05198
• Storage Buffer: 100% PBS pH 7.3
• Storage Conditions: Store at -80°C.