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- KD/KO Validated
EIF2S1 Monoclonal antibody, PBS Only (Capture)
EIF2S1 Monoclonal Antibody for WB, IF/ICC, IP, Cytometric bead array, Indirect ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
WB, IF/ICC, IP, Cytometric bead array, Indirect ELISA
Conjugate
Unconjugated
CloneNo.
3C8E2
Cat no : 68479-1-PBS
Synonyms
Validation Data Gallery
Product Information
68479-1-PBS targets EIF2S1 as part of a matched antibody pair:
MP50181-1: 68479-1-PBS capture and 68023-1-PBS detection (validated in N/A)
Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | EIF2S1 fusion protein Ag28309 |
Full Name | eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa |
Calculated Molecular Weight | 36 kDa |
Observed Molecular Weight | 36 kDa |
GenBank Accession Number | BC002513 |
Gene Symbol | EIF2S1 |
Gene ID (NCBI) | 1965 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS Only |
Storage Conditions | Store at -80°C. |
Background Information
EIF2S1 is one subunit of the translation initiation factor EIF2, which catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. EIF2A (Gene ID: 83939) and EIF2S1 (Gene ID: 1965) share the EIF2A symbol/alias in common. EIF2S1 is the alpha subunit of the eIF2 translation initiation complex. Although both of these proteins function in binding initiator tRNA to the 40S ribosomal subunit, the EIF2A protein does so in a codon-dependent manner, whereas eIF2 complex requires GTP.