EIF2B1 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

EIF2B1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 18010-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of MCF-7 using 18010-1-AP (same clone as 18010-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 18010-1-AP (EIF2B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18010-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 18010-1-AP (same clone as 18010-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 18010-1-AP (EIF2B1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18010-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 18010-1-AP (same clone as 18010-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 18010-1-AP (EIF2B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18010-1-PBS in a different storage buffer formulation.

• 

  IP experiment of K-562 using 18010-1-AP (same clone as 18010-1-PBS)

  IP result of anti-EIF2B1 (IP:18010-1-AP, 4ug; Detection:18010-1-AP 1:700) with K-562 cells lysate 3200ug. This data was developed using the same antibody clone with 18010-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 18010-1-AP (same clone as 18010-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18010-1-AP (EIF2B1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18010-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 18010-1-AP (same clone as 18010-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18010-1-AP (EIF2B1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18010-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 18010-1-AP (same clone as 18010-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18010-1-AP (EIF2B1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18010-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 18010-1-AP (same clone as 18010-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18010-1-AP (EIF2B1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18010-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 18010-1-AP (same clone as 18010-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18010-1-AP (EIF2B1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18010-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 18010-1-AP (same clone as 18010-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18010-1-AP (EIF2B1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18010-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 18010-1-AP (same clone as 18010-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EIF2B1 antibody (18010-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 18010-1-PBS in a different storage buffer formulation.

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Product Information

18010-1-PBS targets EIF2B1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EIF2B1 fusion protein Ag12509
• Full Name: eukaryotic translation initiation factor 2B, subunit 1 alpha, 26kDa
• Calculated Molecular Weight: 305 aa, 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC103763
• Gene Symbol: EIF2B1
• Gene ID (NCBI): 1967
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.