EHMT2 Polyclonal antibody

• Featured Product
• KD/KO Validated

EHMT2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 29303-1-AP

Synonyms

BAT8, C6orf30, DKFZp686H08213, EHMT2, FLJ35547, G9A, H3 K9 HMTase 3, HLA B associated transcript 8, KMT1C, Lysine N methyltransferase 1C, NG36, NG36/G9a, Protein G9a

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Validation Data Gallery

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  WB analysis using 29303-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29303-1-AP (EHMT2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 29303-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29303-1-AP (EHMT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 29303-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29303-1-AP (EHMT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 29303-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 29303-1-AP (EHMT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HepG2 cells, NIH/3T3 cells
• Positive IHC detected in: human lung cancer tissue, human urothelial carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 2 publications below
• IP: See 1 publications below

Product Information

29303-1-AP targets EHMT2 in WB, IHC, IP, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EHMT2 fusion protein Ag29160
• Full Name: euchromatic histone-lysine N-methyltransferase 2
• Calculated Molecular Weight: 1210 aa, 132 kDa
• Observed Molecular Weight: 160-180 kDa
• GenBank Accession Number: BC018718
• Gene Symbol: EHMT2
• Gene ID (NCBI): 10919
• RRID: AB_2918275
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
		Nucleic Acids Res; ATM, KAP1 and the Epstein-Barr virus polymerase processivity factor direct traffic at the intersection of transcription and replication; Authors - Huanzhou Xu
human	WB,IP	Oncogenesis; Calreticulin enhances gastric cancer metastasis by dimethylating H3K9 in the E-cadherin promoter region mediating by G9a.; Authors - Lina Wang ; KD Validated
human	IHC	Mol Biotechnol; Inhibition of the Tumor Suppressor Gene SPINK5 via EHMT2 Induces the Oral Squamous Cell Carcinoma Development; Authors - Suzhen Sun ; KD Validated
human	WB,IHC	Mol Carcinog; A novel high-risk model identified by epithelial-mesenchymal transition predicts prognosis and radioresistance in rectal cancer; Authors - Feiyu Qin